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Team:Wash U/Calendar-Home/1 September 2009
From 2009.igem.org
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The Results of this digest are: | The Results of this digest are: | ||
| - | [[Image:9_1 gel purify.jpg|width=600|height=600 | + | [[Image:9_1 gel purify.jpg]|width=600|height=600] |
The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout | The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout | ||
| + | |||
The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A | The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A | ||
Revision as of 20:31, 1 September 2009
9/1 Minipreps
| number | notation | concentration |
| A | RBS30_cph8 | 168.0 |
| B | ompR_term_ompC_GFP | 161.0 |
| C | ompR_term_ompC_pucB/A | 188.0 |
Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI
Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI.
The Results of this digest are:
[[Image:9_1 gel purify.jpg]|width=600|height=600]
The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout
The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A
