Template:Team:KULeuven/3 September 2009/VanillinProduction

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(Difference between revisions)
(New page: * PCR ** The PCR from yesterday went very well, we had a huge amount of amplified biobrick DNA of the different parts. Agarose gel electrophoresis was used to test if the different pieces ...)
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* PCR
* PCR
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** The PCR from yesterday went very well, we had a huge amount of amplified biobrick DNA of the different parts. Agarose gel electrophoresis was used to test if the different pieces of DNA created by PCR had the correct length.
+
** The PCR from yesterday went very well, we had a huge amount of amplified biobrick DNA of the different parts.
-
** Amplified DNA was purified and cut with different restriction enzymes.
+
Agarose gel electrophoresis was used to test if the different pieces of DNA created by PCR had the correct length.
-
** After the restriction, Sam5, Sam8 and terminator were ligated in a three-way ligation. The same was done for ech, fcs and terminator. We also started a ligation of just Sam5 and Sam8 and ech and fcs.
+
{| border ="1" align="center"
 +
!| gene || concentration || 260/280 || 260/230
 +
|- align="center"
 +
| Sam5 || 361,6 || 1,91 || 2,13
 +
|- align="center"
 +
| Sam8 || 323,8 || 1,92 || 2,06
 +
|- align="center"
 +
| ech || 236,5 || 1,91 || 2,24
 +
|- align="center"
 +
| fcs || 321,4 || 1,90 || 2,22
 +
|-
 +
|}
 +
** Amplified DNA was purified and cut with different restriction enzymes. After restriction, DNA was purified again before ligation.
 +
{| border ="1" align="center"
 +
!| gene || concentration || 260/280 || 260/230
 +
|- align="center"
 +
| Sam5 || 21,8 || 1,65 || 1,33
 +
|- align="center"
 +
| Sam8 || 17,1 || 1,56 || 1,16
 +
|- align="center"
 +
| ech || 13,8 || 1,78 || 1,46
 +
|- align="center"
 +
| fcs || 20,9 || 1,70 || 0,20
 +
|-
 +
|}
 +
** After the restriction, Sam5, Sam8 and terminator were ligated in a three-way ligation. The same was done for ech, fcs and terminator.
 +
We also started a ligation of just Sam5 and Sam8 and ech and fcs.

Revision as of 07:15, 4 September 2009

  • PCR
    • The PCR from yesterday went very well, we had a huge amount of amplified biobrick DNA of the different parts.

Agarose gel electrophoresis was used to test if the different pieces of DNA created by PCR had the correct length.

gene concentration 260/280 260/230
Sam5 361,6 1,91 2,13
Sam8 323,8 1,92 2,06
ech 236,5 1,91 2,24
fcs 321,4 1,90 2,22
    • Amplified DNA was purified and cut with different restriction enzymes. After restriction, DNA was purified again before ligation.
gene concentration 260/280 260/230
Sam5 21,8 1,65 1,33
Sam8 17,1 1,56 1,16
ech 13,8 1,78 1,46
fcs 20,9 1,70 0,20
    • After the restriction, Sam5, Sam8 and terminator were ligated in a three-way ligation. The same was done for ech, fcs and terminator.

We also started a ligation of just Sam5 and Sam8 and ech and fcs.

Retrieved from "http://2009.igem.org/Template:Team:KULeuven/3_September_2009/VanillinProduction"