Team:KULeuven/Wetlab

From 2009.igem.org

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The labwork is divided into four groups, each working on a specific aspect of ''Essencia coli''
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16/07/09 labwerk
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uitplaten glycerol stocks van parts B0015, B0032, J23066, J23110, J23078
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17/07/09
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opzuivering plasmiden uit E. coli cellen.
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[[Image:Missblue-deckchair.png|100px|thumb|left]]
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[https://2009.igem.org/Team:KULeuven/Wetlab/Blue_Light_Receptor '''Blue Light Receptor''']<br>
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Purifying the promotor region and creating it into a new biobrick
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[[Image:Miss_Blue_vanillin_production.png|100px|thumb|left]]
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[https://2009.igem.org/Team:KULeuven/Wetlab/Vanillin_Production '''Vanillin Production''']<br>
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Testing the three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferulic acid to Vanillin) and eventually combining the five enzymes.
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[[Image:Miss_Blue_vanillin_sensor.png|125px|thumb|left]]
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[https://2009.igem.org/Team:KULeuven/Wetlab/Vanillin_Receptor '''Vanillin Receptor''']<br>
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Removing unwanted restriction sites, sequencing and combining all elements into a standard iGEM plasmid.
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[[Image:Miss_Blue_key_lock.png|125px|thumb|left]]
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[https://2009.igem.org/Team:KULeuven/Wetlab/Key_Antikey '''Key/Lock/Antikey''']<br>
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Ligating Key, Lock and Antikey into promotor plasmids and testing the system with a GFP assay.
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Latest revision as of 09:13, 4 September 2009

The labwork is divided into four groups, each working on a specific aspect of Essencia coli

Retrieved from "http://2009.igem.org/Team:KULeuven/Wetlab"