"
EPF-Lausanne/31 August 2009
From 2009.igem.org
(Difference between revisions)
(→Wet Lab) |
|||
| Line 26: | Line 26: | ||
==Wet Lab== | ==Wet Lab== | ||
| + | '''Digestion''' | ||
| + | The Klenow fragments that have been amplified by PCR (after the Klenow reaction) will be digested with SpeI. We have to check if it is indeed the TrpO (presence of the two S sites). | ||
| + | |||
| + | '''Results of the overweekend cultures''' | ||
| + | |||
| + | The idea was to find a new medium without any TRY on it, but on which the cells can still grow. | ||
| + | |||
| + | The minimal media + arginine didn't show any result (nothing grew). | ||
| + | |||
| + | The tubes induced by Try didn't change colour over the week-end. The two that were already red are still red and the others didn't change colour. | ||
| + | |||
| + | ===RO1 construction=== | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 14:39, 4 September 2009
Wet Lab
Digestion
The Klenow fragments that have been amplified by PCR (after the Klenow reaction) will be digested with SpeI. We have to check if it is indeed the TrpO (presence of the two S sites).
Results of the overweekend cultures
The idea was to find a new medium without any TRY on it, but on which the cells can still grow.
The minimal media + arginine didn't show any result (nothing grew).
The tubes induced by Try didn't change colour over the week-end. The two that were already red are still red and the others didn't change colour.
RO1 construction
People in the lab
Basile
