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Team:UNICAMP-Brazil/Notebooks/August 21
From 2009.igem.org
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| - | + | == YeastGuard: Mini-prep, digestion and ligation of RBS == | |
- Today we perfomed mini-prep from the RBS transformed ''E. coli''. The fragment syze was correct,we decided to digest it without purifying and it worked. | - Today we perfomed mini-prep from the RBS transformed ''E. coli''. The fragment syze was correct,we decided to digest it without purifying and it worked. | ||
Revision as of 17:16, 5 September 2009
YeastGuard: Mini-prep, digestion and ligation of RBS
- Today we perfomed mini-prep from the RBS transformed E. coli. The fragment syze was correct,we decided to digest it without purifying and it worked.
- Afetr the RBS plasmid digestion we ligated it to CFP (the reporter gene) according to the protocol W.
Raíssa
