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Template:Team:KULeuven/12 August 2009/BlueLightReceptor
From 2009.igem.org
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JochemDeen (Talk | contribs) (New page: # Gel electrophorese of BlP cut with EcoRI and SpeI # Gel extraction of the Blp Nanodrop results {| border ="1" align="center" !| Part || concentration (ng/μl) || 260/280 λ || 2...) |
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| - | # Gel | + | # Gel electrophoresis of BlP cut with EcoRI and SpeI |
# Gel extraction of the Blp | # Gel extraction of the Blp | ||
Nanodrop results | Nanodrop results | ||
| Line 8: | Line 8: | ||
|- | |- | ||
|} | |} | ||
| - | # PCR (with Pfx) of blue light promoter with primers iGEM 2260 and | + | # PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261 |
#* Annealing of 58C | #* Annealing of 58C | ||
| - | # | + | # Inoculate liquid medium |
| - | #* | + | #* Inoculation of {{kulpart|pSB3K3}}-{{kulpart|BBa_P1010}} and {{kulpart|BBa_J23101}} |
# Ligation | # Ligation | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
| + | |- align="center" | ||
| Vector || insert | | Vector || insert | ||
|- align="center" | |- align="center" | ||
| - | | {{kulpart| | + | | {{kulpart|pSB1A3}}+{{kulpart|BBa_P1010}} || {{kulpart|BBa_K238013}} (BLP) |
|- align="center" | |- align="center" | ||
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl | | 50ng -> 2,5 μl || 5 ng -> 0,3 μl | ||
|- align="center" | |- align="center" | ||
|} | |} | ||
Latest revision as of 08:48, 8 September 2009
- Gel electrophoresis of BlP cut with EcoRI and SpeI
- Gel extraction of the Blp
Nanodrop results
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| Blp | 27 | 2,08 |
- PCR (with Pfx) of blue light promoter with primers iGEM 2260 and iGEM 2261
- Annealing of 58C
- Inoculate liquid medium
- Inoculation of - and
- Ligation
| Vector | insert |
| + | (BLP) |
| 50ng -> 2,5 μl | 5 ng -> 0,3 μl |
