"
Team:KULeuven/17 August 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/BeginHeader}} |
| - | {{Team:KULeuven/Common/ | + | {{Team:KULeuven/Common/SubMenu_Notebook}} |
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/EndHeader}} |
{{Team:KULeuven/Notebook/DayNavigator}} | {{Team:KULeuven/Notebook/DayNavigator}} | ||
| Line 14: | Line 14: | ||
{{Team:KULeuven/EditableTemplateH3|Vanillin Receptor|{{PAGENAME}}/VanillinReceptor}} | {{Team:KULeuven/EditableTemplateH3|Vanillin Receptor|{{PAGENAME}}/VanillinReceptor}} | ||
{{Team:KULeuven/EditableTemplateH3|Key/Lock/Anti-Key|{{PAGENAME}}/KeyLockAntiKey}} | {{Team:KULeuven/EditableTemplateH3|Key/Lock/Anti-Key|{{PAGENAME}}/KeyLockAntiKey}} | ||
| + | {{Team:KULeuven/Common2/PageFooter}} | ||
Latest revision as of 08:48, 10 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
Liquid cultures have been made for the kanamycine resistant plasmid backbone and for the LigA (BLP+ in ).
Lig B has been re-ented on a new plate because too many cells had grown on the one we made on aug 14. We always used single kolonies to re-ent.
[edit] Vanillin Production
- SAMS (ligation sam5+sam8) and EF (ligation ech+fcs): electroporated ...
- and plated out
[edit] Vanillin Receptor
- A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
- Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
- Mutagenesis 2 on R on position 427 was performed with PCR
