Team:Newcastle/Labwork/11 September 2009
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==<u>Metal Sensing Team</u>== | ==<u>Metal Sensing Team</u>== | ||
===Introduction and Outline=== | ===Introduction and Outline=== | ||
- | + | So far we have transformed ''DH5-alpha E. coli'' cells with two of our synthesized BioBricks, which are the ''cotC-GFP-smtA'' and the ''kinA'' BioBricks. The Metal Sensing team have since taken these transformant colonies and used them to set up LB+kanamycin cultures for mini-preps and midi-preps; these were then left overnight in the orbital shaking incubator and are awaiting processing today. However there are a range of tasks which need to be accomplished today; these are listed below: | |
+ | <br> | ||
+ | * '''PCR the ''cotC-GFP-smtA'' BioBrick with ''pMK15'' and ''pMK16''''' | ||
+ | ** Set up the PCR | ||
+ | ** Run the PCR for the correct duration | ||
+ | ** Analyse PCR products through DNA gel electrophoresis | ||
+ | ** Clean up PCR products if PCR reaction proves to be successful | ||
+ | * '''Midi-prep ''cotC-GFP-smtA'', ''kinA'', ''pGFP-rrnB'', ''pMUTIN4'' and ''pSB1AT3''''' | ||
+ | ** Carry out the five midi-preps. | ||
+ | ** Quantify the midi-prep samples | ||
+ | ** Run the midi-prep samples on agarose gel through DNA gel electrophoresis | ||
+ | * Run products from a PCR reaction involving ''arsR'' and ''cadA'' DNA | ||
+ | ** Run PCR products on gel | ||
+ | ** If successful, clean up fragments, run on gel again, cut with restriction enzymes ''NheI'' and ''BamHI'', clean and then ligate together fragments. | ||
+ | <br> | ||
{{:Team:Newcastle/Footer}} | {{:Team:Newcastle/Footer}} | ||
{{:Team:Newcastle/Right}} | {{:Team:Newcastle/Right}} |
Revision as of 23:48, 13 September 2009
Lab Work - 11/09/09
Metal Sensing Team
Introduction and Outline
So far we have transformed DH5-alpha E. coli cells with two of our synthesized BioBricks, which are the cotC-GFP-smtA and the kinA BioBricks. The Metal Sensing team have since taken these transformant colonies and used them to set up LB+kanamycin cultures for mini-preps and midi-preps; these were then left overnight in the orbital shaking incubator and are awaiting processing today. However there are a range of tasks which need to be accomplished today; these are listed below:
- PCR the cotC-GFP-smtA BioBrick with pMK15 and pMK16
- Set up the PCR
- Run the PCR for the correct duration
- Analyse PCR products through DNA gel electrophoresis
- Clean up PCR products if PCR reaction proves to be successful
- Midi-prep cotC-GFP-smtA, kinA, pGFP-rrnB, pMUTIN4 and pSB1AT3
- Carry out the five midi-preps.
- Quantify the midi-prep samples
- Run the midi-prep samples on agarose gel through DNA gel electrophoresis
- Run products from a PCR reaction involving arsR and cadA DNA
- Run PCR products on gel
- If successful, clean up fragments, run on gel again, cut with restriction enzymes NheI and BamHI, clean and then ligate together fragments.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]