Template:Team:KULeuven/12 August 2009/VanillinReceptor

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(New page: * B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commerci...)
 
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* B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
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* B,G and R were stored for further procedure. A failed again: there was no visible growth. This second time there was no problem with the competent cells because we used a commercial product instead of a self made. We assumed there was a problem with adding the polyA-tails to the PCR product because, when redoing the gelelectrophoresis, there were no errors with the PCR product.
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*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene was inserted correctly.
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*PCR was performed for W,X,Y and Z to isolate the genes.

Latest revision as of 09:21, 14 September 2009

  • B,G and R were stored for further procedure. A failed again: there was no visible growth. This second time there was no problem with the competent cells because we used a commercial product instead of a self made. We assumed there was a problem with adding the polyA-tails to the PCR product because, when redoing the gelelectrophoresis, there were no errors with the PCR product.
  • We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene was inserted correctly.
  • PCR was performed for W,X,Y and Z to isolate the genes.
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