Template:Team:KULeuven/13 August 2009/VanillinReceptor

From 2009.igem.org

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# VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA
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# B and R miniprepped. Tomorrow proceed with mutagenesis of ''RpoA''
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# VirG redone (only TOPo was visible, 200bp)  PCR from 2,3 and 1'
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# G redone (only TOPO was visible, 200bp)  PCR from 2,3 and 1'
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# α, β, γ, Δ pc purification to create a TOPO vector tomorow
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# W, X, Y, Z PCR purification to create a TOPO vector tomorrow
Nanodrop:
Nanodrop:
  {| border ="1" align="center"
  {| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
|- align="center"
|- align="center"
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| α || 46,1 || 1,84 ||  
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| W || 46,1 || 1,84 ||  
|- align="center"
|- align="center"
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| β || 112 || 1,82 ||   
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| X || 112 || 1,82 ||   
|- align="center"
|- align="center"
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| γ || 207 || 1,85 ||   
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| Y || 207 || 1,85 ||   
|- align="center"
|- align="center"
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| Δ || 155,3 || 1,86 ||   
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| Z || 155,3 || 1,86 ||   
|-
|-
|}
|}

Latest revision as of 09:22, 14 September 2009

  1. B and R miniprepped. Tomorrow proceed with mutagenesis of RpoA
  2. G redone (only TOPO was visible, 200bp) PCR from 2,3 and 1'
  3. W, X, Y, Z PCR purification to create a TOPO vector tomorrow

Nanodrop:

Part concentration (ng/μl) 260/280 λ 260/230 λ
W 46,1 1,84
X 112 1,82
Y 207 1,85
Z 155,3 1,86
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