Template:Team:KULeuven/10 August 2009/VanillinProduction

From 2009.igem.org

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* Ligated genes are electrophoresed.
* Ligated genes are electrophoresed.
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* Reorderd part I742109 (COMT + RBS) from the registry.
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* Reorderd part {{kulpart|BBa_I742109}} (''COMT'' + RBS) from the registry.
-
* Also we realized the proteins need to be degraded fast for the control mechanism to work, so a degradation tag like LVA is needed.
+
* Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
-
** However since some genes have just been ligated we will only tag Sam5, ComT and the Ech gene.
+
** However since some genes have just been ligated we will only tag ''sam5, COMT'' and the ''ech'' gene.

Latest revision as of 09:54, 14 September 2009

  • Ligated genes are electrophoresed.
  • Reorderd part (COMT + RBS) from the registry.
  • Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
    • However since some genes have just been ligated we will only tag sam5, COMT and the ech gene.