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Template:Team:KULeuven/10 August 2009/VanillinProduction
From 2009.igem.org
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* Ligated genes are electrophoresed. | * Ligated genes are electrophoresed. | ||
| - | * Reorderd part {{kulpart|BBa_I742109}} (COMT + RBS) from the registry. | + | * Reorderd part {{kulpart|BBa_I742109}} (''COMT'' + RBS) from the registry. |
* Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed. | * Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed. | ||
| - | ** However since some genes have just been ligated we will only tag | + | ** However since some genes have just been ligated we will only tag ''sam5, COMT'' and the ''ech'' gene. |
Latest revision as of 09:54, 14 September 2009
- Ligated genes are electrophoresed.
- Reorderd part (COMT + RBS) from the registry.
- Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
- However since some genes have just been ligated we will only tag sam5, COMT and the ech gene.
