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Template:Team:KULeuven/31 August 2009/VanillinProduction
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* Electroporation of SAMS I, SAMS II AND EF II | * Electroporation of SAMS I, SAMS II AND EF II | ||
| - | * Restriction digest of ech and fcs from | + | * Restriction digest of ''ech'' and ''fcs'' from Saturday was tested and showed 6 signals with varying lengths for ''ech'' and 1 very large signal for ''fcs'', both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for'' ech'' and 4 for ''fcs''. |
| - | ** | + | ** Restriction at 37°C overnight. |
| - | * For ligation of SAMS III RD (from | + | * For ligation of SAMS III RD (from Thursday) and TER II we need 44,4 µl of SAMS III which we don't have thus we cut two additional volumes with SAMS I overnight |
** SAMS I A RD and SAMS I B RD | ** SAMS I A RD and SAMS I B RD | ||
Latest revision as of 11:49, 14 September 2009
- Electroporation of SAMS I, SAMS II AND EF II
- Restriction digest of ech and fcs from Saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs.
- Restriction at 37°C overnight.
- For ligation of SAMS III RD (from Thursday) and TER II we need 44,4 µl of SAMS III which we don't have thus we cut two additional volumes with SAMS I overnight
- SAMS I A RD and SAMS I B RD
