Team:Southampton/Notebook

Week Commencing

Summary Of The Week

20th July 2009

‘Brainstorming’ becomes our key word.
We scrutinise various ideas before deciding on ‘Game of Life’ and ‘Rock Paper Scissors’.
Numerous hours were spent on the registry of parts finding approved Biobrick parts.
We become absolute pros at filling out COSHH forms. You just have to work through the cramp.

27th July 2009

Work begins on our wiki page and logo design.
We create a very exciting ‘cluster map’ on our wiki. One cluster so far: Southampton.
We finalise our plasmid diagrams and Biobrick parts.
The team mugshot was taken.
We went from lab protocol amateurs to experts within the week.
The team supervisor abandoned us for 7 days. ‘Family holiday’ was the excuse. We soldiered on.

3rd August 2009

The hard works begins…
DNA Biobricks were transformed, starter cultures made and plasmid maxiprep performed.
Our Southampton iGEM logo is nearly finalised. 
Cluster map update: 9 different hits including Canada, Turkey, Poland, Japan and Netherlands.
Work commences on the simulations.
One man down :( . Apparently going to hospital is the new excuse for getting out of labs.

10th August 2009

Man back :) , he just could not stay away.
Note to self:
1) In the plasmid maxiprep: Turns out it does make a difference if you accidently put the white column on top of the blue, instead of the other way round…
2) Adding B0034 to B0015 and centrifuging them together does not, in fact, bode well.
3) The nanodrop only works when the switch is turned to ‘on’.
Exciting times, having been all maxiprepped out, we started a new protocol: Digestion.
After much deliberation and input from the entire team, our logo is finally finished. It was worth the wait. I mean look at it. Genius.
Cluster map update:
UK (i.e. Southampton) – 57
Rest of world – 30
Still a bit of room for improvement…

17th August 2009

We are granted permission to start an outreach project …hold tight folks, more details coming soon.
Our team diminishes further - apparently ‘working at home’ is the new ‘working…at work’.
Our PCRs FINALLY work. Maybe practise really does make perfect.
We perform our last ever Maxiprep…sob.
Don’t panic though, we are moving onto bigger and better protocols; Ligation.
It’s nearly week six, and we have finally mastered the art of making and loading a gel. It’s ‘simple’ they say. Obviously not.
An exciting wall chart appears in our office.
Cluster map update: UK – 92. Really must stop checking the cluster map so often…

25th August 2009

We perfect a pattern:
Digest…ligate…transform
Digest…ligate…transform
Digest…ligate…transform
Shame none of them worked…
On the plus side, our simulations are
looking good!

31st August 2009

The team begins to feel pretty
demoralised as the second week of
‘digest…ligate…transform…FAIL’
commences.
We decide to momentarily focus on building our ‘novel’ biobrick.
Good news though, our simulations
specific to RPS/GoL are complete. Now
to make them applicable to
anything/everything else!
Work starts on our synthetic biology
video…soon to be the most demanded
on YouTube.
Our wiki page has been transformed.
Just you wait for the launch next week.
Mesmerising.
We are warned about round two of
abandonment by team supervisor.
‘Climbing’ is the new excuse. Not even
blaming the family this time!

7th September 2009

We think we have finally optimised our
digest…ligate technique. However,
when we come to transform, we have a
distinct lack of competent cells. Whose
authorisation do we need? The project
supervisor. And where is he? Turkey.
Splendid.
Instead, we concentrate on more
‘behind the scenes’ such as content for
our wiki. It’s going well, and we are
collecting information fast.
Progress is also being made with
regard to the video, the script is almost
complete, and filming is due to start
early next week. Now, who wants to be
in it?
To fill the lack of time spent in the labs,
we decided to do a ‘fun’ side project, to
optimize SYBR gold gels. It failed. Yes,
an experiment with no use other than
to fill time, failed. Time to go home…