Team:Chiba

From 2009.igem.org

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Revision as of 13:36, 16 September 2009

The Project

Introduction

Project Design

Conclusions

Delay Switch

Experiments 2008

Results 2008

Experiments 2009

Results 2009

Parallel Activation

Modeling

Experiments

Results

Series Activation

Modeling

Experiments

Results

E.coli Time Manager Since 2008

Welcome to the Chiba team Wiki for iGEM 2009!

Team Photo

I’ll introduce Team Chiba to you. Team Chiba joined iGEM in 2006. Its members belong to The Dep. of Chemistry and so we did not know much biology. The members therefore had to start from studying the basics of biology. This was the characteristic, but what I believe to be the biggest weak point for the Team.

This year, However, Team Chiba has a member who belongs to the Dep. of biology! He is nothing but an undergraduate student but I expect that the energy of our team will be made better by his existence.

This years project is making the most of last year's project. Last year, we tried to devise a series of delay-switches by designing a "switching consortia". We got limited, in success in generating delay swithces, but I think that there is a great deal of room for improvement.

The Registration of our team is very late, in June, and our team has only 5 people. I don't know what will become of our team, but I think the best for our team that all members our team enjoy to the full! Thank you. (Asia iGEM Workshop)

Project - E. coli Time Manager Since 2008

What we working since 2008 is to make a function of "Demonstrate its role in a group" timer.A concrete system is the one of causing the color change because "Receiver cell" perceives the signal molecule that "Transmitter cell" makes. The color change occurs when the signal molecule increases gradually and it reaches the density that the reception cell finally feels.The term that necessary to this accumulation becomes the time of the timer.

We devised to make "Felt time" shared in tens of thousands of cells in the system by using the molecule that was able to pass the cell membrane freely(Asyl-Homoserine Lactone(AHL) groups) as a signal molecule.As a result, cells can act the action at the same time in good order in splinters as the group, not begin to be "Shift" shines.

This year's purpose is to to make the timer that functions to several step floor by using a new timer cell with built-in this "Match at time" function.

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 == Project - ''E''. coli Time Manager Since 2008 ==
-We are constructing delay switches to control/preset the timing of target gene expression.
-Our project uses two classes of bacteria: senders and receivers. Senders produce signaling molecules, and receivers are activated only after a particular concentration of this molecule is reached. The combinatorial use of senders/receivers allows us to make a‘switching consortium’which activates different genes at the preset times.
 What we working since 2008 is to make a function of "Demonstrate its role in a group" timer.A concrete system is the one of causing the color change because "Receiver cell" perceives the signal molecule that "Transmitter cell" makes. The color change occurs when the signal molecule increases gradually and it reaches the density that the reception cell finally feels.The term that necessary to this accumulation becomes the time of the timer.