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Team:Warsaw/Calendar-Main/13 May 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3>Testing of Biobrick parts obtained from registry</h3> <h4>Marek</h4> <ol><li>Minipreps of Biobrick DNA.</li> <li>Restriction diges...) |
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| - | <h3>Testing of Biobrick parts obtained from registry</h3> | + | <h3>Testing of Biobrick parts obtained from the registry</h3> |
<h4>Marek</h4> | <h4>Marek</h4> | ||
<ol><li>Minipreps of Biobrick DNA.</li> | <ol><li>Minipreps of Biobrick DNA.</li> | ||
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<p>Gel electrophoresis of digests</p> | <p>Gel electrophoresis of digests</p> | ||
<img src="https://static.igem.org/mediawiki/2009/a/a0/Zel_meagan_bricki.jpg"/> | <img src="https://static.igem.org/mediawiki/2009/a/a0/Zel_meagan_bricki.jpg"/> | ||
| + | <var> | ||
| + | <p>Proper insert sizes:</p> | ||
| + | <ol> | ||
| + | <li>C0051 - 750bp <b>OK</b></li> | ||
| + | <li>R0010 - 200 bp <b>?</b></li> | ||
| + | <li>C008- - 915 bp <b>OK</b></li> | ||
| + | <li>E0026 - 762 bp <b>OK</b></li> | ||
| + | <li>R0080 - 149 bp <b>?</b></li> | ||
| + | <li>C0040 - 660 bp <b>OK</b></li> | ||
| + | <li>I759002 - 312 bp <b>OK</b></li> | ||
| + | <li>E0040 - 720 bp <b>OK</b></li> | ||
| + | <li>B0010 - 80 bp <b>?</b></li> | ||
| + | <li>J06505 - 723 bp <b>?</b></li> | ||
| + | </ol> | ||
| + | </var> | ||
| + | <h4>Kamil</h4> | ||
| + | <ol><li>Restriction digest of minipreps with EcoRI and PstI enzymes</li> | ||
| + | <li>Electrophoresis carried out on 1,5% agarose gel</ol> | ||
| + | <img src="https://static.igem.org/mediawiki/2009/thumb/0/02/2009.05.13.jpg/800px-2009.05.13.jpg" width="65%" heigth="65%"/> | ||
| + | <ul><li>Most of the bricks are to small to be observed on the gel and should be tested by direct sequencing. </li><li>The E0032 brick seems fine, the I14032 looks puzzling and should also undergo sequencing.</li></ul> | ||
</html> | </html> | ||
Latest revision as of 17:44, 17 September 2009
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Testing of Biobrick parts obtained from the registry
Marek
- Minipreps of Biobrick DNA.
- Restriction digest of minipreps with EcoRI and PstI
Milena
Gel electrophoresis of digests
Proper insert sizes:
- C0051 - 750bp OK
- R0010 - 200 bp ?
- C008- - 915 bp OK
- E0026 - 762 bp OK
- R0080 - 149 bp ?
- C0040 - 660 bp OK
- I759002 - 312 bp OK
- E0040 - 720 bp OK
- B0010 - 80 bp ?
- J06505 - 723 bp ?
Kamil
- Restriction digest of minipreps with EcoRI and PstI enzymes
- Electrophoresis carried out on 1,5% agarose gel
- Most of the bricks are to small to be observed on the gel and should be tested by direct sequencing.
- The E0032 brick seems fine, the I14032 looks puzzling and should also undergo sequencing.
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