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Team:Chiba/Notebook/Calendar/17 September 2009
From 2009.igem.org
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==Digestion Test== | ==Digestion Test== | ||
| + | 14:30~ | ||
| + | Double Digestion | ||
| + | {| border="1" align="center" | ||
| + | | || volume (uL) | ||
| + | |- align="center" | ||
| + | | DNA || 3 (300ng) | ||
| + | |- align="center" | ||
| + | | EcoRI || 0.20 (4U) | ||
| + | |- align="center" | ||
| + | | PstI || 0.20 (4U) | ||
| + | |- align="center" | ||
| + | | Buffer(10x) || 1 | ||
| + | |- align="center" | ||
| + | | BSA(10x)|| 1 | ||
| + | |- align="center" | ||
| + | | dW || 4.6 | ||
| + | |- align="center" | ||
| + | | total || 10 | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | Single Digestion | ||
| + | {| border="1" align="center" | ||
| + | | || volume (uL) | ||
| + | |- align="center" | ||
| + | | DNA || 3 (300ng) | ||
| + | |- align="center" | ||
| + | | EcoRI || 0.20 (4U) | ||
| + | |- align="center" | ||
| + | | Buffer(10x) || 1 | ||
| + | |- align="center" | ||
| + | | dW || 5.8 | ||
| + | |- align="center" | ||
| + | | total || 10 | ||
| + | |} | ||
| + | |||
| + | |||
| + | |||
| + | Gel | ||
| + | |||
| + | agar(low electro-osmosis)---0.7%(W)---0.42 g | ||
| + | |||
| + | TAE---60 ml | ||
== Lab Notebook == | == Lab Notebook == | ||
Revision as of 04:19, 23 September 2009
(16_September_2009 <|>18_September_2009)
Mini Prep
10:30~
K091118(1985bp) pSB1A2(2079bp)
K091134(2048bp) pSB1AK3(3189bp)
S03956(1041bp) pSB1A2
I731012(1930bp) pSB1A2
Digestion Test
14:30~
Double Digestion
| volume (uL) | |
| DNA | 3 (300ng) |
| EcoRI | 0.20 (4U) |
| PstI | 0.20 (4U) |
| Buffer(10x) | 1 |
| BSA(10x) | 1 |
| dW | 4.6 |
| total | 10 |
Single Digestion
| volume (uL) | |
| DNA | 3 (300ng) |
| EcoRI | 0.20 (4U) |
| Buffer(10x) | 1 |
| dW | 5.8 |
| total | 10 |
Gel
agar(low electro-osmosis)---0.7%(W)---0.42 g
TAE---60 ml
Lab Notebook
