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Team:DTU Denmark/project
From 2009.igem.org
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| - | < | + | <font size="4"><b>Project abstract</b></font><br><br> |
| + | <font size="3"><b>The Redoxilator</b></font><br> | ||
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| + | <p align="justify">By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast.</p> | ||
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| + | <font size="3"><b>The USER fusion standard</b></font><br> | ||
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| + | <p align="justify">Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions.</p> | ||
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Revision as of 17:42, 23 September 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
|
The redoxilator - Theoretical background - Yeast as a model organism - Practical approach The USER assembly standard - Principle - Proof of concept - Manual - Primer design software |
DTU denmark Project abstract The Redoxilator By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD/NADH redox poise. A future novel application of the system is heterologous redox coupled protein production in yeast. The USER fusion standard Another part of our project is the proposal of a new parts-assembly standard for Biobricks based on USER(TradeMark) cloning. With this technique, not based on restriction enzymes, all parts independent of function can be assembled without leaving any scars from the restriction enzyme digestions. |
Medal status We surely have the gold by now! |
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