"
Team:NCTU Formosa/Project/Introduction
From 2009.igem.org
Poiuytrechu (Talk | contribs) |
Poiuytrechu (Talk | contribs) |
||
| Line 158: | Line 158: | ||
#nctucontent { width:830px; margin:0px auto;} | #nctucontent { width:830px; margin:0px auto;} | ||
#nctucontent1 { width:780px; margin:40px 0px 0px 20px; font-family:Arial, Helvetica, sans-serif; } | #nctucontent1 { width:780px; margin:40px 0px 0px 20px; font-family:Arial, Helvetica, sans-serif; } | ||
| - | #intitle { width:770px; } | + | #intitle { width:770px; margin-bottom:10px;} |
| - | #innerbox { width:770px; } | + | #innerbox { width:770px; margin-top:10px;} |
#innertext { font-size:12px; } | #innertext { font-size:12px; } | ||
#innerimg {border:2px #cccccc solid; background:#f9f9f9; } | #innerimg {border:2px #cccccc solid; background:#f9f9f9; } | ||
| Line 261: | Line 261: | ||
<div id="innertext"> | <div id="innertext"> | ||
<span style="font-size:1.4em">Bacterial referee with the adjustable timer and counter functions</span> | <span style="font-size:1.4em">Bacterial referee with the adjustable timer and counter functions</span> | ||
| - | <p>Our team constructed a controllable synthetic genetic circuit in <b><i>Escherichia coli</i> which has timer and counter functions</b>. The circuit works as an OR gate to integrate temporal and environmental signals.</p> | + | <br><br><p>Our team constructed a controllable synthetic genetic circuit in <b><i>Escherichia coli</i> which has timer and counter functions</b>. The circuit works as an OR gate to integrate temporal and environmental signals.</p><br> |
| - | <p>The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After <i>E.coli</i> consume the added lactose, the RFP will be translated to remind us that time’s up.</p> | + | <p>The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After <i>E.coli</i> consume the added lactose, the RFP will be translated to remind us that time’s up.</p><br> |
| - | <p>The counter function can detect the bacteria population with LuxI/LuxR device; Moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.</p> | + | <p>The counter function can detect the bacteria population with LuxI/LuxR device; Moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.</p><br> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 11:29, 24 September 2009
Introduction
Our team constructed a controllable synthetic genetic circuit in Escherichia coli which has timer and counter functions. The circuit works as an OR gate to integrate temporal and environmental signals.
The output (red fluorescent protein: RFP) of the OR gate is ON when one of the input signals is ON. The timer function is controlled by Lac promoter, and the concentration of lactose determines timer’s working length. After E.coli consume the added lactose, the RFP will be translated to remind us that time’s up.
The counter function can detect the bacteria population with LuxI/LuxR device; Moreover, the counter sensitivity is controlled by the strength of TetR repressible promoter. If external bacteria invade the system, the extra AHL produced by them will induce the RFP translation to warn us the contamination. Our project can be applied to storage warning signs of fresh food, contact lens, and wound dressing.
