Team:Chiba/Notebook/Calendar/24 September 2009

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(→Examine limit of AHL generation)
(→Examine limit of AHL generation)
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*Today's operation
*Today's operation
-
22:00
+
14:00
-
We measured OD.
+
We made plates from each of mixture.
-
OD<sub>600</sub>=1.98
 
 +
*Element of mixtures
-
Then, we spun down this culture solution and add 12.5 mL of flesh LB-Amp.
+
<table width="900" border="1" cellpadding="0"  cellspacing="0" bordercolor="#000000"><tr>
-
 
+
<td width="100">Sample Namber</td>
-
 
+
<td width="100">1</td>
-
22:10
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<td width="100">2</td>
-
 
+
<td width="100">3</td>
-
We add 12.5 &mu;L of IPTG to the culture solution and started culture at 37 degrees Celsius.
+
<td width="100">4</td>
 +
<td width="100">5</td>
 +
<td width="100">6</td>
 +
<td width="100">7</td>
 +
<td width="100">8</td>
 +
</tr>
 +
<tr>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>20 &mu;L</td>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>2</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>3</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>4</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>5</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>6</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>---</td>
 +
<td>5 mL</td>
 +
<td>---</td>
 +
</tr>
 +
<tr>
 +
<td>LB-Amp,Cm</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>9.8 mL</td>
 +
</tr>
 +
<tr>
 +
<td>Total</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
<td>10 mL</td>
 +
</tr>
 +
</table>

Revision as of 15:40, 25 September 2009

>Go to the Notebook page

(23_September_2009 <|>25_September_2009)


Examine limit of AHL generation

Yesterday's operation is here.


  • Today's operation

14:00

We made plates from each of mixture.


  • Element of mixtures
Sample Namber 1 2 3 4 5 6 7 8
--- 5 mL --- --- --- --- --- --- 20 μL
1 --- 5 mL --- --- --- --- --- ---
2 --- --- 5 mL --- --- --- --- ---
3 --- --- --- 5 mL --- --- --- ---
4 --- --- --- --- 5 mL --- --- ---
5 --- --- --- --- --- 5 mL --- ---
6 --- --- --- --- --- --- 5 mL ---
LB-Amp,Cm 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 9.8 mL
Total 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL 10 mL
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