Team:Chiba/Notebook/Calendar/26 September 2009

From 2009.igem.org

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Revision as of 02:54, 27 September 2009

>Go to the Notebook page

(25_September_2009 <|>27_September_2009)


Contents

E.coli Painting(3)

RFP, GFP. CFP

Examine limit of AHL generation(2)-2

Yesterday's operation is here.


  • Today's operation

11:35-

We transplanted E.coli to NC filter and put these on each plates.

And then we took pictures every 30 min.


Picture are here.


Pictures

11:35

Negative Control

1

2

3

4

5

6

7

8


12:05

Negative Control

1

2

3

4

5

6

7

8


12:35

Negative Control

1

2

3

4

5

6

7

8


13:05

Negative Control

1

2

3

4

5

6

7

8


13:35

Negative Control

1

2

3

4

5

6

7

8


14:05

Negative Control

1

2

3

4

5

6

7

8


14:35

Negative Control

Chiba DSCF0549.jpg

1

2

3

4

5

6

7

8


15:05

Negative Control

1

2

3

Chiba DSCF0561.jpg

4

5

6

7

8


15:35

Chiba DSCF0567.jpg

Negative Control

1

Chiba DSCF0569.jpg

2

Chiba DSCF0570.jpg

3

Chiba DSCF0571.jpg

4

Chiba DSCF0572.jpg

5

Chiba DSCF0573.jpg

6

Chiba DSCF0574.jpg

7

Chiba DSCF0575.jpg

8

Making colors

PCR

  • Template

1 : ECFP

2 : mCherry

3 : V-YFP

4 : mRFP without Nco1 site

5 : mOrange

6 : sfGFP


14:45

We drew up mixtures for PCR.


  • Element of mixtures
Template 1 μL
Primer(F) 2.5 μL
Primer(R) 2.5 μL
Buffer(x 10) 5 μL
dNTP 4 μL
DNA pol. 1 μL
dH2O 34 μL
Total 50 μL


15:20-

PCR


Gel electrophoresis

15:30

We made gel for electrophoresis.

agar 0.14 g

TAE 20 mL


17:25-

Gel electrophoresis(135 V, 27 min)

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/26_September_2009"