Team:Chiba/Project/Delay Switch
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(→Exp #3' LuxR Mutants (One More Plan : Direct Screening for Delay Switcher)) |
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=== Experiments (2009) === | === Experiments (2009) === | ||
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- | ==== | + | ==== Exp #3' LuxR Mutants (One More Plan : Direct Screening for Delay Switcher) ==== |
:In this plan, we introduced the random mutation into the entire reading frame of LuxR using error-prone PCR. | :In this plan, we introduced the random mutation into the entire reading frame of LuxR using error-prone PCR. | ||
+ | The mutants plasmid would be transformed with LuxP-gfp plasmid into E coli. Colonies were lifted onto nitrocellulose filter and placed on the top of lawn of Lux-senders. Over time, we kept watching the colony hue. Colonies should turn green when they detect the message passing through the NC filter. We were looking for the colony that turns green significantly after those of wildtype. | ||
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=== Results (2009) === | === Results (2009) === |
Latest revision as of 15:23, 27 September 2009
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E.coli Time Manager Since 2008
Constructing A Delay Switch Since 2008In principle, there are three ways to delay the activation of chemical communications;
Inter-species communications! We decided to go for the strategy inspired by Japanese-classic experience; Whenever we speak to somebody in English, we often experience a certain delay in activating the communication. We though this is exactly what we pursued in. See Exp #4. Same applies to the reverse, too. When somebody speaks to us, we definitely need some time (sometime infinite) to get activated. This is all in spite that he/ she was loud and clear enough. The less affinity (perception) we have to English, the longer we need to activate them.See exp #5. Experiments (2008)For details, click each of the index titles
Results (2008)In conclusion, we tried (and are trying) to device a series of delay-switches by designing the "switching consortia". We got limited, by certain success in generating delay switches. Experiments (2009)
Results (2009) |