"
Team:DTU Denmark/parts
From 2009.igem.org
| Line 187: | Line 187: | ||
<p align="justify">C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.</p> | <p align="justify">C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.</p> | ||
| - | Part id: <a href="http://partsregistry.org/Part:BBa_K194000" >BBa_K194000</a> | + | Part id: <a href="http://partsregistry.org/Part:BBa_K194000" target="_blank">BBa_K194000</a> |
<br> | <br> | ||
<br> | <br> | ||
| Line 194: | Line 194: | ||
<p align="justify">Green Fluorescent Protein (GFP) codonoptimized for yeast. </p> | <p align="justify">Green Fluorescent Protein (GFP) codonoptimized for yeast. </p> | ||
| - | Part id: <a href="http://partsregistry.org/Part:BBa_K194001" >BBa_K194001</a> | + | Part id: <a href="http://partsregistry.org/Part:BBa_K194001" target="_blank">BBa_K194001</a> |
<br> | <br> | ||
<br> | <br> | ||
| Line 201: | Line 201: | ||
<p align="justify">This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.</p> | <p align="justify">This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.</p> | ||
| - | Part id: <a href="http://partsregistry.org/Part:BBa_K194002" >BBa_K194002</a> | + | Part id: <a href="http://partsregistry.org/Part:BBa_K194002" target="_blank">BBa_K194002</a> |
<br> | <br> | ||
<br> | <br> | ||
Revision as of 13:02, 1 October 2009
| Home | The Team | The Project | Parts submitted | Modelling | Notebook |
|
Also check out our Danish website DTU iGEM 2009 |
Parts submitted to registry cln2 PEST destabilization domain for rapid protein turnover C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes. Part id: BBa_K194000GFP (a yeast- and FACS-optimized GFP variant) Green Fluorescent Protein (GFP) codonoptimized for yeast. Part id: BBa_K194001Destabilized GFP for yeast This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes. Part id: BBa_K194002 |
Facts about parts No facts yet |
| Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us |
