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Imperial College London/Notebook/23 September 2009
From 2009.igem.org
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**Cannot really conclude that the increase of fluorescence is due to an increase of expression as opposed to simple cell growth. This can be seen by the similarity of data on the normalised graph (bottom right, figure 1). | **Cannot really conclude that the increase of fluorescence is due to an increase of expression as opposed to simple cell growth. This can be seen by the similarity of data on the normalised graph (bottom right, figure 1). | ||
| - | [[Image:II09_HVD-GFP-run1.png]] | + | [[Image:II09_HVD-GFP-run1.png|300px]] |
Revision as of 14:41, 1 October 2009
Nuri: - Plotted curves GFP/normalized OD - Observations: Control is dodgy... negative values came up, so need to re-do in wetlab! - Diauxic growth model: Writing up and reviewing model and simulations
Testing team
Harvard GFP (1st run)
- Conclusions:
- Raw fluorescence seems to increase with time at 37°C while, at 28°C, it seems to stay stable
- Problems:
- Did not run enough data points to see the fluorescence of the shifted sample reach a steady state
- Cannot really conclude that the increase of fluorescence is due to an increase of expression as opposed to simple cell growth. This can be seen by the similarity of data on the normalised graph (bottom right, figure 1).
