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Team:Chiba/Notebook/Calendar/30 September 2009
From 2009.igem.org
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== Transformation == | == Transformation == | ||
| - | Yesterday's operation is [ here]. | + | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here]. |
| + | |||
| + | |||
| + | *Today's operation | ||
| + | 10:30- | ||
| + | |||
| + | Culture at 37 degrees Celsius | ||
| + | |||
| + | |||
| + | 20:00- | ||
| + | |||
| + | Mini Prep. | ||
| + | |||
| + | |||
| + | 20:40- | ||
| + | |||
| + | Digestion Test | ||
| + | |||
| + | |||
| + | 20:56- | ||
| + | |||
| + | At 37 degrees Celsius | ||
| + | |||
| + | |||
| + | 21:45- | ||
| + | |||
| + | Gel electro...(135 V, 25 min) | ||
| + | |||
| + | |||
| + | |||
| + | == PCR == | ||
| + | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here]. | ||
| + | |||
| + | 11:15- | ||
| + | |||
| + | Gel electro...(135 V, 27 min) | ||
| + | |||
| + | |||
| + | 12:10 | ||
| + | |||
| + | Took a picture | ||
| + | |||
| + | == Cloning == | ||
| + | === Digestion === | ||
| + | Yesterday's operation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/29_September_2009 here]. | ||
| + | |||
| + | 12:37- | ||
| + | |||
| + | Gel electro...(100 V, 40 min) | ||
| + | |||
| + | |||
| + | 13:20 | ||
| + | |||
| + | Ethyl bromideに浸す | ||
| + | |||
| + | |||
| + | 13:35 | ||
| + | |||
| + | Took a picture and ゲルを切り出す。 | ||
| + | |||
| + | Then we added 2 mL of ADB Buffer and put it in 37 degrees Celsius of 培養機 | ||
| + | |||
| + | |||
| + | === DNA Clean & Concentrator === | ||
| + | We 溶出ed 50 μL. | ||
| + | |||
| + | |||
| + | |||
| + | === SAP処理 === | ||
| + | *まぜ表 | ||
| + | |||
| + | |||
| + | 15:45- | ||
| + | |||
| + | インキュベート it at 37 degrees Celsius. | ||
| + | |||
| + | |||
| + | ---30 min--- | ||
| + | |||
| + | |||
| + | |||
| + | 16:20 | ||
| + | |||
| + | We transferred it at 65 degrees Celsius and 失活させた | ||
| + | |||
| + | |||
| + | ---15 min--- | ||
| + | |||
| + | |||
| + | |||
| + | === DNA Clean & Concentrator === | ||
| + | We 溶出ed 20 μL. | ||
| + | |||
| + | |||
| + | |||
| + | === Gel electro... === | ||
| + | 17:32 | ||
| + | |||
| + | Electro... | ||
| + | |||
| + | |||
| + | 18:15 | ||
| + | |||
| + | Took a picture | ||
| + | |||
| + | |||
| + | |||
| + | === Ligation === | ||
| + | 18:30 | ||
| + | |||
| + | @Room Temperture | ||
| + | |||
| + | |||
| + | 21:30 | ||
| + | |||
| + | Transformation | ||
| + | |||
| + | |||
| + | 21:50 | ||
| + | |||
| + | @37 degrees Celsius | ||
| + | |||
| + | |||
| + | 23:10 | ||
| + | |||
| + | Plating | ||
Revision as of 08:43, 3 October 2009
(29_September_2009 <|>1_October_2009)
Contents |
Transformation
Yesterday's operation is here.
- Today's operation
10:30-
Culture at 37 degrees Celsius
20:00-
Mini Prep.
20:40-
Digestion Test
20:56-
At 37 degrees Celsius
21:45-
Gel electro...(135 V, 25 min)
PCR
Yesterday's operation is here.
11:15-
Gel electro...(135 V, 27 min)
12:10
Took a picture
Cloning
Digestion
Yesterday's operation is here.
12:37-
Gel electro...(100 V, 40 min)
13:20
Ethyl bromideに浸す
13:35
Took a picture and ゲルを切り出す。
Then we added 2 mL of ADB Buffer and put it in 37 degrees Celsius of 培養機
DNA Clean & Concentrator
We 溶出ed 50 μL.
SAP処理
- まぜ表
15:45-
インキュベート it at 37 degrees Celsius.
---30 min---
16:20
We transferred it at 65 degrees Celsius and 失活させた
---15 min---
DNA Clean & Concentrator
We 溶出ed 20 μL.
Gel electro...
17:32
Electro...
18:15
Took a picture
Ligation
18:30
@Room Temperture
21:30
Transformation
21:50
@37 degrees Celsius
23:10
Plating
