Template:Team:KULeuven/Components/Key Lock Antikey

From 2009.igem.org

(Difference between revisions)

Latest revision as of 10:39, 4 October 2009

Key Lock Antikey

The key/antikey system compares the signal from the blue light and vanillin receptor in order to control vanillin production. The more the measured amount of vanillin exceeds the wanted amount (set by the blue light intensity), the less vanillin is produced. After blue light irradiation, the key is transcribed and ‘unlocks’ the vanillin synthesis pathway. In response to the produced vanillin, the vanillin receptor then activates transcription of the antikey. Key and antikey are complementary RNA-strands: their annealing is favoured over the reaction between key en lock leading to vanillin synthesis.

@@ Line 1: / Line 1: @@
-{{Team:KULeuven/Components/Component|9/96/Miss_Blue_key_lock.png|Key Lock Antikey|The key and antikey system perform a subtraction of the blue light signal and the vanillin receptor signal. The result controls the vanillin production. In biological terms, the subtraction translates as the annealing of complementary RNA strands, the key and the antikey. This reaction is favoured over the reaction between the key and the lock leading to vanillin synthesis. In this way we try to perform the subtraction before inducing production of vanillin.
+{{Team:KULeuven/Components/Component|9/96/Miss_Blue_key_lock.png|Key Lock Antikey}}
+<html><p></html>
-The biological equivalent of a subtraction can only yield a positive number, so one can only subtract a small from a large amount. Because we can only actively produce vanillin, we have to subtract the measured quantity of vanillin from the wanted quantity, or in other words, the amount of antikey produced by the vanillin receptor from the amount of key produced by the blue light sensor.}}
+The key/antikey system compares the signal from the blue light and vanillin receptor in order to control vanillin production. The more the measured amount of vanillin exceeds the wanted amount (set by the blue light intensity), the less vanillin is produced. After blue light irradiation, the key is transcribed and ‘unlocks’ the vanillin synthesis pathway. In response to the produced vanillin, the vanillin receptor then activates transcription of the antikey. Key and antikey are complementary RNA-strands: their annealing is favoured over the reaction between key en lock leading to vanillin synthesis.
+<html></p></html>