EPF-Lausanne/11 September 2009

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Latest revision as of 12:48, 4 October 2009

Wet Lab

O.D. for the Trp K.O. strains

For the competent cells !

After 5p.m. :

JRG1046 : 1.35. JRG465 : 0.85. JW4356-2 : 1.64. JW4356-2 (Kana) : 1.57.

For today we drop the experiment, we are too far from 0.6. We will do it on Monday.

Results of the characterization

RO2-BB #1, all clones were illuminated :

RO2-BB#1 plot

We have induction (repression repressed...) from LovTAP, we have to investigate further to be sure it is what we want.

Tryptophan concentration experiment on RO2 :

RO2#4 TRP plot

All the clones react in the same way, here it is the graph for RO2#4. It seems we have an increase until somewhere between 2ul and 4 ul (in 50 ul of culture, TRP : 4.25 g/L).

RO2#5

RO2#5 plot

RO2#10

RO2#10 plot

RO1-BB didn't respond.

RO2#4, influence of TRP and ATC

File:RO24TRPATC.jpg

RO2#4 TRP ATC plot

Nicolas ce graph n'est pas dans ce que tu nous a envoyé, est-ce que tu peux le rajouter stp ???

Read out 1 characterization

To summarize our results, we did the following graphic :

RO1 final plot

We can say that Read Out 1 is characterized, it shows exactly the answer we would expect : if we add TRP, there is a significant decrease in RFP expression.

People in the lab

Mélanie, Caroline, Basile, Nicolas

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@@ Line 26: / Line 26: @@
 ==Wet Lab==
+===O.D. for the Trp K.O. strains===
+For the competent cells !
+After 5p.m. :
+JRG1046 : 1.35. JRG465 : 0.85. JW4356-2 : 1.64. JW4356-2 (Kana) : 1.57.
+For today we drop the experiment, we are too far from 0.6. We will do it on Monday.
+===Results of the characterization===
+RO2-BB #1, all clones were illuminated :
+[[Image:RO2-BB1.jpg‎|center|thumb|upright=4|RO2-BB#1 plot]]
+We have induction (repression repressed...) from LovTAP, we have to investigate further to be sure it is what we want.
+Tryptophan concentration experiment on RO2 :
+[[Image:RO24TRP.jpg‎|center|thumb|upright=4|RO2#4 TRP plot]]
+All the clones react in the same way, here it is the graph for RO2#4. It seems we have an increase until somewhere between 2ul and 4 ul (in 50 ul of culture, TRP : 4.25 g/L).
+RO2#5
+[[Image:RO25.jpg‎|center|thumb|upright=4|RO2#5 plot]]
+RO2#10
+[[Image:RO210.jpg‎|center|thumb|upright=4|RO2#10 plot]]
+RO1-BB didn't respond.
+RO2#4, influence of TRP and ATC
+[[Image:RO24TRPATC.jpg‎|center|thumb|upright=4|RO2#4 TRP ATC plot]]
+Nicolas ce graph n'est pas dans ce que tu nous a envoyé, est-ce que tu peux le rajouter stp ???
+===Read out 1 characterization===
+To summarize our results, we did the following graphic :
+[[Image:RO1final.jpg‎|center|thumb|upright=4|RO1 final plot]]
+We can say that Read Out 1 is characterized, it shows exactly the answer we would expect : if we add TRP, there is a significant decrease in RFP expression.
 ==People in the lab==
@@ Line 35: / Line 68: @@
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