Team:Freiburg bioware/Testpage

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<h1>Team Freiburg Bioware</h1>
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<br />
 +
<p>The first German team ever to participate in iGEM is back
 +
again and
 +
after last year's second place we're highly motivated &nbsp;to make
 +
some good piece of synthetic biology in 2009. In this year we want to
 +
create an universal restriction enzyme to facilitate labwork
 +
and enable new techniques. <br />
 +
<br />
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We're looking forward to meeting you on this year's jamboree!<br />
 +
</p>
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<h2 class="art-PostHeaderIcon-wrapper"> <span
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class="art-PostHeader">Project Summary</span> </h2>
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</div>
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<div class="art-PostContent"><span
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class="art-button-wrapper"></span><b><br />
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Universal Endonuclease &ndash; Cutting Edge Technology</b>
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<div class="art-PostContent">
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<p>Gene technology is driven by the use of restriction
 +
endonucleases.
 +
Yet, constraints of limited sequence length and variation recognized by
 +
available restriction enzymes pose a major roadblock for synthetic
 +
biology. We developed the basis for universal restriction enzymes,
 +
primarily for routine cloning but also with potential for in vivo
 +
applications. We use a nucleotide cleavage domain fused to a binding
 +
domain, which recognizes a programmable adapter that mediates binding
 +
to DNA and thus cleavage. As adapter we use readily available modified
 +
oligonucleotides, as binding domain anticalins and as cleavage domain
 +
FokI moieties engineered for heterodimerization and activity. For
 +
cloning, this universal enzyme has merely to be mixed with the sequence
 +
specific oligonucleotide and the target DNA. Binding and release are
 +
addressed with thermocycling. Additionally, we provide concepts for in
 +
vivo applications by external adapter delivery, activity regulation by
 +
photo-switching, as well as for modifying an argonaute protein towards
 +
a DNA endonuclease.
 +
</p>
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<div class="t">FREiGEM 2009</div>
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<p><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span
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style="font-weight: bold;"><img
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style="border: 0px solid ; width: 138px; height: 40px;"
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alt="Team"
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src="https://static.igem.org/mediawiki/2009/f/f6/Freiburg09_Team.gif" /></span></a><br />
 +
<b>The Team</b><br />
 +
In 2009 our team consists of &nbsp;14 undergraduates and 4 advisors.<br />
 +
<a href="https://2009.igem.org/Team:Freiburg_bioware/Team">Read
 +
more...</a></p>
 +
<p><span style="font-weight: bold;"><a
 +
href="http://www.bioss.uni-freiburg.de"><img
 +
style="border: 0px solid ; width: 144px; height: 48px;"
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Bioss</span><br />
 +
We want to thank our main sponsor Bioss for supporting our project.<br />
 +
<a href="http://www.bioss.uni-freiburg.de">Read more...</a></p>
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Revision as of 13:19, 4 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Artisteer

Welcome

an image

Team Freiburg Bioware


The first German team ever to participate in iGEM is back again and after last year's second place we're highly motivated  to make some good piece of synthetic biology in 2009. In this year we want to create an universal restriction enzyme to facilitate labwork and enable new techniques.

We're looking forward to meeting you on this year's jamboree!


Project Summary


Universal Endonuclease – Cutting Edge Technology

Gene technology is driven by the use of restriction endonucleases. Yet, constraints of limited sequence length and variation recognized by available restriction enzymes pose a major roadblock for synthetic biology. We developed the basis for universal restriction enzymes, primarily for routine cloning but also with potential for in vivo applications. We use a nucleotide cleavage domain fused to a binding domain, which recognizes a programmable adapter that mediates binding to DNA and thus cleavage. As adapter we use readily available modified oligonucleotides, as binding domain anticalins and as cleavage domain FokI moieties engineered for heterodimerization and activity. For cloning, this universal enzyme has merely to be mixed with the sequence specific oligonucleotide and the target DNA. Binding and release are addressed with thermocycling. Additionally, we provide concepts for in vivo applications by external adapter delivery, activity regulation by photo-switching, as well as for modifying an argonaute protein towards a DNA endonuclease.

FREiGEM 2009

Team
The Team
In 2009 our team consists of  14 undergraduates and 4 advisors.
Read more...

bioss
Bioss
We want to thank our main sponsor Bioss for supporting our project.
Read more...

Visitors

 freigem09@googlemail.com

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