Team:UNIPV-Pavia/Methods Materials/Absorbance

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(Absorbance and diluitions in liquid growing medium)
(Absorbance and diluitions in liquid growing medium)
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The proportionality between absorbance measurement (OD600) and colture's diluitions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well.  
The proportionality between absorbance measurement (OD600) and colture's diluitions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well.  
This experiment was done in two different growing mediums: LB and M9.
This experiment was done in two different growing mediums: LB and M9.
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This experiment was done in two different growing mediums: LB and M9.
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You can find more informations about the experiments at LINK - Test n.20, 13/08/09 and Test n.20 "M9", 28/08/09.
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[[Image:Unipv_asb_diluition_LB.jpg| 500px|Absorbance and colture's diluitions in LB|thumb|center|Absorbance and colture's diluitions in LB]]
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[[Image:unipv_asb_diluition_M9.jpg| 500px|Absorbance and colture's diluitions in M9|thumb|center|Absorbance and colture's diluitions in M9]]

Revision as of 11:44, 6 October 2009

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Measurements - Absorbance

Absorbance and water dispensation

We find no relevant differences between the measures before and after the dispensation of different volumes of water in the well, both for bacterial coltures and simple liquid growing medium.

You can find more informations about the experiment at LINK - Test n.9, 07/07/09.


Absorbance and water dispensation


Absorbance and volume

The proportionality between absorbance (OD600) measurement and volume of colture in the well was verified.

You can find more informations about the experiment at LINK - Test n.9, 07/07/09.


Absorbance and volume


Absorbance and diluitions in liquid growing medium

The proportionality between absorbance measurement (OD600) and colture's diluitions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well. This experiment was done in two different growing mediums: LB and M9.


This experiment was done in two different growing mediums: LB and M9.

You can find more informations about the experiments at LINK - Test n.20, 13/08/09 and Test n.20 "M9", 28/08/09.


Absorbance and colture's diluitions in LB
Absorbance and colture's diluitions in M9