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Team:UNIPV-Pavia/Methods Materials/Fluorescence
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Differently from the measures of absorbance, we find out that the dispensation of water in the well causes an amplification of the fluorescence signal, both for bacterial coltures and simple liquid growing medium (in figure the results for a colture producing GFP). | Differently from the measures of absorbance, we find out that the dispensation of water in the well causes an amplification of the fluorescence signal, both for bacterial coltures and simple liquid growing medium (in figure the results for a colture producing GFP). | ||
| - | This amplification seams to be non linear, if analised on a large scale of volumes. | + | This amplification seams to be non linear and characterized by a kind of saturation, if analised on a large scale of volumes. |
You can find more informations about the experiment at LINK - Test n.9, 07/07/09. | You can find more informations about the experiment at LINK - Test n.9, 07/07/09. | ||
Revision as of 14:43, 6 October 2009
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Measurements - Fluorescence |
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Fluorescence and water dispensationDifferently from the measures of absorbance, we find out that the dispensation of water in the well causes an amplification of the fluorescence signal, both for bacterial coltures and simple liquid growing medium (in figure the results for a colture producing GFP). This amplification seams to be non linear and characterized by a kind of saturation, if analised on a large scale of volumes. You can find more informations about the experiment at LINK - Test n.9, 07/07/09.
 Fluorescence and diluitions in waterFluorescence and volumeFluorescence and diluitions in liquid growing mediumFluorescence and evaporation |
