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Team:UNIPV-Pavia/Methods Materials/Fluorescence
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[[Image:Unipv_fu_diluition_M9.jpg| 500px|Fluorescence and diluitions M9|thumb|center|Fluorescence and diluitions LB]] | [[Image:Unipv_fu_diluition_M9.jpg| 500px|Fluorescence and diluitions M9|thumb|center|Fluorescence and diluitions LB]] | ||
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== <html><font class="dayw_style">Bleaching in fluorescent coltures</font></html> == | == <html><font class="dayw_style">Bleaching in fluorescent coltures</font></html> == | ||
Revision as of 15:46, 6 October 2009
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Measurements - Fluorescence |
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Fluorescence and water dispensationDifferently from the measures of absorbance, we find out that the dispensation of water in the well causes an amplification of the fluorescence signal, both for bacterial coltures and simple liquid growing medium (in figure the results for a colture producing GFP). This amplification seams to be non linear and characterized by a kind of saturation, if analised on a large scale of volumes. You can find more informations about the experiment at LINK - Test n.9, 07/07/09.
Fluorescence and diluitions in waterThe proportionality between fluorescence measurements (GFP and RFP) and different diluitions of LB was verified in two volumetric diluitions. This considerations has not to be read together with the results of the non-liner relationship between fluorescence and water dispensation, because even if different amounts of water in the well means different diluition of the colture, in that simulation we didn't work with costant volume in the well, as in this case we do. The first two measurements must not be taken into account because of the high measure's rumour. You can find more informations about the experiment at LINK - Test n.18, 06/08/09.
Fluorescence and volumeThe relationship between fluorescence (both GFP and RFP) and volume of LB in the well present a non-linear characteristic. This test will be done also using bacterial coltures, because we note that the behaviour of the signal from fluorescent proteins is different from that coming from the autofluorescence. You can find more informations about the experiment at LINK - Test n.14, 30/07/09.
 Fluorescence and diluitions in liquid growing medium  Temporal evolution of fluorescence measurementsBleaching in fluorescent coltures
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