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Team:Washington/Accomplishments
From 2009.igem.org
| Line 18: | Line 18: | ||
BBa_K215000 | BBa_K215000 | ||
| - | + | * Protein Expression Vector | |
BBa_K215001 | BBa_K215001 | ||
| - | + | * Fusion protein sequence | |
This part is used in: | This part is used in: | ||
BBa_K215010 | BBa_K215010 | ||
| - | + | * GFP fusion protein | |
BBa_K215011 | BBa_K215011 | ||
| - | + | * BBa_K215090 (organic phosphotriesterase) fusion protein | |
BBa_K215091 | BBa_K215091 | ||
| Line 34: | Line 34: | ||
Future directions: | Future directions: | ||
| - | + | * Make more universal, to work with all proteins | |
| - | + | * Optimize secretion-tag and nano-tag for fusion proteins | |
| - | + | ||
[[image:Secretion_structure.png |300px]] | [[image:Secretion_structure.png |300px]] | ||
| Line 50: | Line 49: | ||
* Gene encoding prtF | * Gene encoding prtF | ||
BBa_K215103 | BBa_K215103 | ||
| - | * BBa_K21500 + BBa_K21501 | + | * BBa_K21500 + BBa_K21501 prtD + prtE |
BBa_K215104 | BBa_K215104 | ||
| - | * BBa_K25103 + BBa_K25102 | + | * BBa_K25103 + BBa_K25102 prtD + prtE + prtF |
BBa_K215105 | BBa_K215105 | ||
| - | * BBa_K215104 + BBa_B0014 in PSB1AK3 | + | * BBa_K215104 + BBa_B0014 in PSB1AK3 prtDEF + Terminator |
BBa_K215106 | BBa_K215106 | ||
| - | * BBa_K215104 + BBa_B0014 in PSB3T5 | + | * BBa_K215104 + BBa_B0014 in PSB3T5 prtDEF + Terminator |
BBa_K215108 | BBa_K215108 | ||
| - | * BBa_J23114 + BBa_K215106 | + | * BBa_J23114 + BBa_K215106 Medium Promoter + prtDEF-Terminator |
BBa_K215109 | BBa_K215109 | ||
| - | * BBa_J23113 + BBa_K215106 | + | * BBa_J23113 + BBa_K215106 Weak Promoter + prtDEF-Terminator |
Current Status: In progress | Current Status: In progress | ||
Revision as of 03:20, 8 October 2009
BBa_K215002 Is a BioBrick part that was made to work in conjunction with the Secretion System (BBa_K215107). When used it creates a fusion protein, that contains the prtB secretion signal recognized by the secretion system. It also fuses to the protein a nano-tag that is able to bind Streptavidin, TEV protease site (for isolating your protein from the fusion) and Histidine Epitote Tags for easy protein purification.
This part is composed of:
BBa_K215000
- Protein Expression Vector
BBa_K215001
- Fusion protein sequence
This part is used in:
BBa_K215010
- GFP fusion protein
BBa_K215011
- BBa_K215090 (organic phosphotriesterase) fusion protein
BBa_K215091
Current Status: Complete
Future directions:
- Make more universal, to work with all proteins
- Optimize secretion-tag and nano-tag for fusion proteins
BBa_K215107 Is a BioBrick version of the Type 1 secretion system of Erwinia Chrysanthemi. It secretes proteins that contain the prtB C-terminus Epitote tag. To create a fusion protein with the prtB tag see BBa_K215002.
This part is composed of: BBa_K215100
- Gene encoding prtD
BBa_K215101
- Gene encoding prtE
BBa_K215102
- Gene encoding prtF
BBa_K215103
- BBa_K21500 + BBa_K21501 prtD + prtE
BBa_K215104
- BBa_K25103 + BBa_K25102 prtD + prtE + prtF
BBa_K215105
- BBa_K215104 + BBa_B0014 in PSB1AK3 prtDEF + Terminator
BBa_K215106
- BBa_K215104 + BBa_B0014 in PSB3T5 prtDEF + Terminator
BBa_K215108
- BBa_J23114 + BBa_K215106 Medium Promoter + prtDEF-Terminator
BBa_K215109
- BBa_J23113 + BBa_K215106 Weak Promoter + prtDEF-Terminator
Current Status: In progress
Future directions:
- Optimize RBS's
- Move secretion sequence into new plasmid, not TetR
- Try variety of cell lines.
BBa_K215200
BBa_K215201
BBa_K215210
BBa_K215211
BBa_K215250
BBa_K215251
BBa_K215260
BBa_K215261
Current Status: In progress
Future Directions:
- Use new CDS display vector to display streptavidin
- Design monomeric protein to bind peptide sequence
- Utilize other proteins on CDS to test for activity of other proteins
| Biobricks Submitted | Current Status Report | Future Directions |
|---|
Quick overview of what was done and the status of everyting. Maybe 3 paragraphs (display, secretion, conclusions)
