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EPF-Lausanne/7 October 2009
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Miniprep of the Trp-mutated strains that should be double-transformants (RO1.1+BB1 and RO2.4+BB1) to extract the DNA and make a digestion assay in order to confirm the gel's results. Also miniprepped DH5 alpha RO2.4+BB1 clone n.3 to extract the 2 working plasmids (LovTap and read-out 2), so that we can use this DNA directly for future transformations. | Miniprep of the Trp-mutated strains that should be double-transformants (RO1.1+BB1 and RO2.4+BB1) to extract the DNA and make a digestion assay in order to confirm the gel's results. Also miniprepped DH5 alpha RO2.4+BB1 clone n.3 to extract the 2 working plasmids (LovTap and read-out 2), so that we can use this DNA directly for future transformations. | ||
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| + | ===Results of the plate-reader experiment=== | ||
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| + | 071009_dh5alpha_ro2_dt.jpg | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 07:31, 8 October 2009
Wet Lab
Prepared cells for time-course experiment:
- 7mL of fresh LB + 1mL of overnight cell culture (DH5 alpha RO2.4 + BB1 clone n.3)
- 5 different conditions:
- +light +IPTG -Trp
- +light -IPTG -Trp
- -light +IPTG -Trp
- -light -IPTG +Trp
- -light -IPTG -Trp
The cells were put in the incubator at 37°C in their respective experimental conditions. Measurements of OD and fluorescence will be taken every 30 min starting at 1h of incubation, so that we have a time-course measurement. For the last sample, we will do a kinetic measurement overnight to see the decay of the RFP fluroescence.
Miniprep of the Trp-mutated strains that should be double-transformants (RO1.1+BB1 and RO2.4+BB1) to extract the DNA and make a digestion assay in order to confirm the gel's results. Also miniprepped DH5 alpha RO2.4+BB1 clone n.3 to extract the 2 working plasmids (LovTap and read-out 2), so that we can use this DNA directly for future transformations.
Results of the plate-reader experiment
071009_dh5alpha_ro2_dt.jpg
People in the lab
Gab, Tu
