"
His+FluA+SplitLinker+Foki in pMA Vector
1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa
r w h f s g k c a r n p y l f i f l n t
61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt
f k y v s a h e t i t l i n a s i i l k
121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa
k e e y e y s t f p c r p y s l f c g i
>>........................AmpR..........................>
m s i q h f r v a l i p f f a a f
181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca
l p s c f c s p r n a g e s k r c - r s
>.............................AmpR..............................>
c l p v f a h p e t l v k v k d a e d q
241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa
v g c t s g l h r t g s q q r - d p - e
>.............................AmpR..............................>
l g a r v g y i e l d l n s g k i l e s
301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc
f s p r r t f s n d e h f - s s a m w r
>.............................AmpR..............................>
f r p e e r f p m m s t f k v l l c g a
361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct
g i i p y - r r a r a t r s p h t l f s
>.............................AmpR..............................>
v l s r i d a g q e q l g r r i h y s q
421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt
e - l g - v l t s h r k a s y g w h d s
>.............................AmpR..............................>
n d l v e y s p v t e k h l t d g m t v
481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact
k r i m q c c h n h e - - h c g q l t s
>.............................AmpR..............................>
r e l c s a a i t m s d n t a a n l l l
541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt
d n d r r t e g a n r f f a q h g g s c
>.............................AmpR..............................>
t t i g g p k e l t a f l h n m g d h v
601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt
n s p - s l g t g a e - s h t k r r a -
>.............................AmpR..............................>
t r l d r w e p e l n e a i p n d e r d
661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat
h h d a c s n g n n v a q t i n w r t t
>.............................AmpR..............................>
t t m p v a m a t t l r k l l t g e l l
721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg
y s s f p a t i n r l d g g g - s c r t
>.............................AmpR..............................>
t l a s r q q l i d w m e a d k v a g p
781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg
t s a l g p s g w l v y c - - i w s r -
>.............................AmpR..............................>
l l r s a l p a g w f i a d k s g a g e
841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
caccaagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc
a w f s r y h c s t g a r w - a l p y r
>.............................AmpR..............................>
r g s r g i i a a l g p d g k p s r i v
901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct
s y l h d g e s g n y g - t k - t d r -
>.............................AmpR..............................>
v i y t t g s q a t m d e r n r q i a e
961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa
d r c l t d - a l v t v r p s l l i y t
>............AmpR............>>
i g a s l i k h w -
1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat
l d - f k t s f l i - k d l g e d p f -
1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc
- s h d q n p l t - v f v p l s v r p r
1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt
r k d q r i f l r s f f s a r n l l l a
1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa
n k k t t a t s g g l f a g s r a t n s
1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg
f s e g n w l q q s a d t k y c s s s v
1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt
a v v r p p l q e l c s t a y i p r s a
1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt
n p v t s g c c q w r - v v s y r v g l
1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg
k t i v t g - g a a v g l n g g f v h t
1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt
a q l g a n d l h r t e i p t a - a m r
1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt
k r h a s r r e k g g q v s g k r q g r
1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc
n r r a h e g a s r g k r l v s l - s c
1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg
r v s p p l t - a s i f v m l v r g a e
1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac
p m e k r q q r g l f t v p g l l l a f
1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga
gagtaatccg tggggtccga aatgggcttg ctggctcgcg tcgctcagtc actcgctcct
c s l g t p g f t r t t e r s e s v s e
1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga
tcgcctctcg cgggttatgc gttcctttgt cgatactggt acaattacgt cgaccgtgct
e a e s a q y a r k q l - p c - c s w h
1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag
gtccaaaggg ctgacctttc gcccgtcact cgccttccgg gtactccggt caattaattc
d r f p d w k a g s e r k a h e a s - l
NotI
--+------
EcoRI XbaI NgoMIV
-+----- -+---- -+----
1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc
tccatggctt aagcgccggc gaagatctac cggccggtag tagtagtagt agtatggccg
r g t e f a a a s r w p a i i i i i i p
>>.............ORF-1...............>
m a g h h h h h h t g
>>....His Tag.....>>
h h h h h h
2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc
ctgcacatgg tgctgccgcg gacggggctt cacttcgggc acctgttgaa gctgaccagg
a t c t t t a p a p k - s p w t t s t g
>.............................ORF-1.............................>
d v y h d g a c p e v k p v d n f d w s
>>............................FluA..............................>
d v y h d g a c p e v k p v d n f d w s
2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc
gtcatggtgc cgttcaccac cctccaccgg ttcatggggt cggggttgcc gttcataccg
p s t t a s g g r w p s t p a p t a s m
>.............................ORF-1.............................>
q y h g k w w e v a k y p s p n g k y g
>.............................FluA..............................>
q y h g k w w e v a k y p s p n g k y g
2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac
ttcacgccga cccggctcat gtgggggctc ccgttctcgc acttccacag gtctatgctg
a s a a g p s t p p r a r a - r c p d t
>.............................ORF-1.............................>
k c g w a e y t p e g k s v k v s r y d
>.............................FluA..............................>
k c g w a e y t p e g k s v k v s r y d
2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag
cactaggtgc cgtttcttat gaagtacctt ccgtggcgga tggggcaccc gctgtcgttc
t - s t a k n t s w k a p p t p w a t a
>.............................ORF-1.............................>
v i h g k e y f m e g t a y p v g d s k
>.............................FluA..............................>
v i h g k e y f m e g t a y p v g d s k
2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac
tagccgttct agatggtgtc ggcctggcac ccgccgatgt ggtctttctg gcacaagttg
r s a r s t t a g p w a a t p e r p c s
>.............................ORF-1.............................>
i g k i y h s r t v g g y t r k t v f n
>.............................FluA..............................>
i g k i y h s r t v g g y t r k t v f n
2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac
cacgacaggt ggctgttgtt cttgatgtag tagccgatgt cgacggcgat gctgctcctg
t c c p p t t r t t s s a t a a a t t r
>.............................ORF-1.............................>
v l s t d n k n y i i g y s c r y d e d
>.............................FluA..............................>
v l s t d n k n y i i g y s c r y d e d
2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag
ttcttcccgg tgaccctggt gcacacccac gacagggcca ggtaccacga ctggccgctc
t r r a t g t t c g c c p g p w c - p a
>.............................ORF-1.............................>
k k g h w d h v w v l s r s m v l t g e
>.............................FluA..............................>
k k g h w d h v w v l s r s m v l t g e
2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg
cggttctggc ggcacctctt gatggactag ccgtcggggc accacctgtc ggtctttgac
r p r p p w r t t - s a a p w w t a r n
>.............................ORF-1.............................>
a k t a v e n y l i g s p v v d s q k l
>.............................FluA..............................>
a k t a v e n y l i g s p v v d s q k l
2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt
cacatgtcgc tgaagtcgct ccggcggacg tttcacttgt tgtggccggc tggtcggaca
w c t a t s a r p p a k - t t p a d q p
>.............................ORF-1.............................>
v y s d f s e a a c k v n n t g r p a c
>....................FluA...................>>
v y s d f s e a a c k v n n
Split-Linker >>..........>
r p a c
2581 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag
ttctaaggtt tactggactt cgtctttcaa tacttagtgt ggccgtttag acttgacctc
v r f q m t - s r k l - i t p a n l n w
>.............................ORF-1.............................>
k i p n d l k q k v m n h t g k s e l e
>..............Split-Linker.............>>
k i p n d l k q k v m n h
>>.'Fok inaktiv'..>
g k s e l e
2641 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg
ctctttttta ggctcgacgc ggtgtttgac tttatacacg gagtgctcat atagcttgac
r r k n p s c a t n - n m c l t s i s n
>.............................ORF-1.............................>
e k k s e l r h k l k y v p h e y i e l
>.........................'Fok inaktiv'.........................>
e k k s e l r h k l k y v p h e y i e l
2701 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc
tagctctagc gggcattatc atgggttctg gcataggacc tttactttca ctacctcaag
- s r s p v i v p k t v s w k - k - w s
>.............................ORF-1.............................>
i e i a r n s t q d r i l e m k v m e f
>.........................'Fok inaktiv'.........................>
i e i a r n s t q d r i l e m k v m e f
2761 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt
aagtactttc agataccgat agcaccgttt gtagacccac catcggcatt tggtcgtcca
s s - k s m a i v a n i w v v a v n q q
>.............................ORF-1.............................>
f m k v y g y r g k h l g g s r k p a g
>.........................'Fok inaktiv'.........................>
f m k v y g y r g k h l g g s r k p a g
2821 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat
cggtaaatat ggcaaccaag gggctagcta ataccgcaat agcaccggtg ttttcgcata
v p f i p l v p r s i m a l s w p q k r
>.............................ORF-1.............................>
a i y t v g s p i d y g v i v a t k a y
>.........................'Fok inaktiv'.........................>
a i y t v g s p i d y g v i v a t k a y
2881 tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag
agaccgccaa tattagacgg ctaaccagtc cgactactct accttgcaat acaccttctc
i l a v i i c r l v r l m r w n v m w k
>.............................ORF-1.............................>
s g g y n l p i g q a d e m e r y v e e
>.........................'Fok inaktiv'.........................>
s g g y n l p i g q a d e m e r y v e e
2941 aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt
ttagtctggg cattgtttgt agacttgggc ttgcttacca cctttcacat aggcagttca
r i r p v t n i - t r t n g g k c i r q
>.............................ORF-1.............................>
n q t r n k h l n p n e w w k v y p s s
>.........................'Fok inaktiv'.........................>
n q t r n k h l n p n e w w k v y p s s
3001 gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag
cagtggctca agtttaaaga caagcactcg ccggtgaaat ttccgttgat atttcgggtc
v s p s s n f c s - a a t l k a t i k p
>.............................ORF-1.............................>
v t e f k f l f v s g h f k g n y k a q
>.........................'Fok inaktiv'.........................>
v t e f k f l f v s g h f k g n y k a q
3061 ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg
gactgagcag acttggtata gtggttatcg ttaccccgtc acgactcaca actccttgac
s - l v - t i s p i a m g q c - v l r n
>.............................ORF-1.............................>
l t r l n h i t n s n g a v l s v e e l
>.........................'Fok inaktiv'.........................>
l t r l n h i t n s n g a v l s v e e l
3121 ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa
gactagccac ctctttacta gtttcgtccg tgggactgag accttcttca agcggcattt
c - s v e k - s k q a p - l w k k f a v
>.............................ORF-1.............................>
l i g g e m i k a g t l t l e e v r r k
>.........................'Fok inaktiv'.........................>
l i g g e m i k a g t l t l e e v r r k
PstI
------+
SpeI NotI
-+---- --+-----
3181 ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca
aagttgttac cgctctagtt aaaatggcca attatgatca tcgccggcga cgtctcgagt
n s t m a r s i l p v n t s s g r c r a
>..............ORF-1..............>>
f n n g e i n f t g -
>..........'Fok inaktiv'.........>>
f n n g e i n f t g
3241 tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt
accgcgcgga tccggaactg ccggaaggcg gttaagcggg atatcactca gcataatgca
h g a p r p - r p s a n s p y s e s y y
3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
gcgcgagtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg
v a l t g r r f t t s - l g k p w r y p
3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt
t - s p c s t s p f r q l a - - r r g p
3421 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg
ggctttgcgg gaagggttgt caacgcgtcg gacttaccgc ttaccctcgc gggacatcgc
h r n a l p n s c a a - m a n g s a l -
3481 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
cggtgagttg ggatagagcc agataagaaa actaaatatt ccctaaaacg gctaaagccg
r p l n p i s v y s f d l - g i l p i s
3541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
gataaccaat tttttactcg actaaattgt ttttaaattg cgcttaaaat tgttttataa
a y w l k n e l i - q k f n a n f n k i
3601 aacgcttaca atttag
ttgcgaatgt taaatc
l t l t i -
FASTA sequence<h3> >His+FluA+Split+FokI_pMA Ligation of His+FluA+Split+FokI into pMA2_NgoMIV_SpeI GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAAT GCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTG TCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACG AGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTT TTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTA TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCG CCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAG AAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGG AGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAA CTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACT ATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACT GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCG CGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAG TTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCA AGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTA AAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCT TAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAA AGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAA CAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTA CCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAA TACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTG TAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCT GCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTT ACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGC CCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAG CTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCC GGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGG GAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTT GAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAA CGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG CTCATTAGGCACCCCAGGCTTTACCCGAACGACCGAGCGCAGCGAGTCAG TGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAAACAGCTATGACCA TGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA GCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCGAATTCGCGGCCG CTTCTAGATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACC ACGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCC CAGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGG CAAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCG TGAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAA GGCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAG CCGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCA CCGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGAC AAGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCT GACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCG TGGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGC AAAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAA GCAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAAT CCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTG ATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGT GATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTG GTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGAT TATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCC GATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCC GTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGT GTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTA TAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAG TGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGC ACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAA TTTTACCGGTTAATACTAGTAGCGGCCGCTGCAGAGCTCATGGCGCGCCT AGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGT CGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGC GTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG TAATAGCGAAGAGGCCCGCACCGAAACGCCCTTCCCAACAGTTGCGCAGC CTGAATGGCGAATGGGAGCGCCCTGTAGCGGCCACTCAACCCTATCTCGG TCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTA AAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATT AACGCTTACAATTTAG
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