Team:Alberta/References/Title

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     <h1>BioBytes</h1>
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     <h1>References</h1>
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<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> -->
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<b>NEED TO PROPERLY FORMAT REFERENCES</b>
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<div align="justify">
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<font size="2">Team BioBytes is the University of Alberta's 2009 International Genetically Engineered Machines (iGEM) team. This year's iGEM project can be subdivided into two major efforts. The first and most important of which is the BioBytes chromosome assembly system. This system refers to a mechanism for rapid and reliable construction of plasmids (i.e.: artificial gene sets) in vitro. The second, the minimal genome project, refers to the ultimate goal of rapid and reliable DNA assembly, that is, the construction of an artificial <i>E. coli</i> chromosome. Furthermore, it includes the strategy of gene selection, arrangement, artificial chromosome insertion and the destruction of the host's chromosome.</P>
 
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      </div></div>
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<p>A family of LIC vectors for high through-put cloning and purification of proteins Eschenfeldt (2009)</p>
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<b class="b4f"></b><b class="b3f"></b><b class="b2f"></b><b class="b1f"></b>
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<p>An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red Fukiya (2004)</p>
-
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
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    <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
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    <div class="Overview">
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    <div style="height: 800; background:#FFFFFF; line-height:100% padding: 3px 0px;">
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    <h2>BioBytes Chromosome Assembly</h2>
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<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> -->
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<p>The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame Flower (1986)</p>
-
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<P>Current methods for DNA assembly are incredulously slow and complicated, and tend to break down with large scale additions. The current method, in brief, is to incorporate genes constructed in the form of "bricks", put them into a helper plasmid, amplify them within the bacterial host <i>E. coli</i>, and purify them before beginning the next cycle of additions. At each addition step, the construct must be structurally and functionally evaluated. The entire cycle is routinely completed over three to four days, while troubleshooting and correction require considerable effort. It is also pertinent to know that the system has a tendency to break down after five or more additions.
+
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</P><P>
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-
These issues have left a void in genetic engineering that the BioBytes chromosome assembly system hopes to fill. Our system involves the linking of Streptavidin to a carefully structured set of magnetic beads. Streptavidin strongly binds to biotin, a moiety that can be synthetically added to double stranded DNA molecules. This Bead-Streptavidin-Biotin-Double Stranded DNA platform provides a secure base for genetic information to be added sequentially. Genes are to be created in a standardized format so that they can added one after another, with each gene binding to the last, to form a long chain of DNA as per Figure 1 (See below). This is a far simpler method than the current method of inserting single genes into an already circular piece of DNA. In one final step, the single stranded DNA is released from the biotin and recircularized with the end of the final brick. This is illustrated in Figure 2 (See below). The BioBytes chromosome assembly system requires ~15 minutes per gene addition cycle, a marked improvement over the current three day methods.</P>
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<img src="https://static.igem.org/mediawiki/2009/a/ac/UofA09_overview_F1.jpg">
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Figure 1: A means for linking consecutive genes using the BioBytes chromosome assembly system.
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<img src="https://static.igem.org/mediawiki/2009/1/1b/UofA09_overview_F2.jpg">
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Figure 2: A means for recircularizing the artificially constructed chromosome once all the desired bricks have been sequentially added.
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</font>
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      </div></div>
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<p>Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation Lu (2006)</p>
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    </td>
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  </tr>
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<tr>
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<p>Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size Huang (1996)</p>
-
<td style="height: 800; padding-left: 10px; padding-right: 10px; padding-top: 11px;">
+
 
-
    <b class="b1f"></b><b class="b2f"></b><b class="b3f"></b><b class="b4f"></b>
+
<p>Biochemistry of homologous recombination in Escherichia coli Kowalczykowski (1994)</p>
-
    <div class="Overview">
+
 
-
    <div style="height: 800; background:#FFFFFF; line-height:100% padding: 3px 0px;">
+
<p>BioWarehouse: a bioinformatics database warehouse toolkit Lee (2006)</p>
-
    <h2>The Minimal Genome Project</h2>
+
 
 +
<p>Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome Hashimoto (2005)</p>
 +
 
 +
<p>Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t Ellwood (1982)</p>
 +
 
 +
<p>Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector Shizuya (1992)</p>
 +
 
 +
<p>Coding-sequence determinants of gene expression in Escherichia coli Kudla (2009)</p>
 +
 
 +
<p>Compilation and analysis of Escherichia coli promoter DNA sequences Hawley (1983)</p>
 +
 
 +
<p>Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium Gibson (2008)</p>
 +
 
 +
<p>Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research Kitagawa (2005)</p>
 +
 
 +
<p>Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria Haldimann (2001)</p>
 +
 
 +
<p>Conjugal Mobilization of Plasmid DNA: Termination Frequency at the Origin of Transfer of Plasmid R1162 Rao (1994)</p>
 +
 
 +
<p>Control of rRNA Synthesis in Escherichia coli: a Systems Biology Approach Dennis (2004)</p>
 +
 
 +
<p>Construction of consecutive deletions of the Escherichia coli chromosome Kato (2007)</p>
 +
 
 +
<p>Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection Baba (2006)</p>
 +
 
 +
<p>Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome Kato (2008)</p>
 +
 
 +
<p>Determination of the core of a minimal bacterial gene set Gil (2004)</p>
 +
 
 +
<p>DNA cloning by homologous recombination in Escherichia coli Zhang (2000)</p>
 +
 
 +
<p>DNA Replication Kornberg (1988)</p>
 +
 
 +
<p>DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine Kelman (1995)</p>
 +
 
 +
<p>Dynamic strength of molecular adhesion bonds Evans (1997)</p>
 +
 
 +
<p>Effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages, The Davison (1959)</p>
 +
 
 +
<p>Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12 Hasunuma (1979)</p>
 +
 
 +
<p>Effect of shear on plasmid DNA in solution Levy (1999)</p>
 +
 
 +
<p>Emergent properties of reduced genome Escherichia coli Posfai (2006)</p>
 +
 
 +
<p>Engineering a reduced Escherichia coli genome Kolisnychenko (2002)</p>
 +
 
 +
<p>Engineering BioBrick vectors from BioBrick parts Shetty (2008)</p>
 +
 
 +
<p>Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon Nomura (1999)</p>
 +
 
 +
<p>Escherichia coli and its chromosome Reyes-Lamothe (unknown)</p>
 +
 
 +
<p>Escherichia coli physiology in Luria-Bertani broth Sezonov (2007)</p>
 +
 
 +
<p>Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665 Gerdes (2003)</p>
 +
 
 +
<p>Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson (2009)</p>
 +
 
 +
<p>Fermentation pathways of Escherichia coli, The Clark (1989)</p>
 +
 
 +
<p>Folding and unfolding single RNA molecules under tension Woodside (2008)</p>
 +
 
 +
<p>Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations Siegele (1997)</p>
 +
 
 +
<p>Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli Herring (2007)</p>
 +
 
 +
<p>Gene splicing and mutagenesis by PCR driven overlap extension Heckman (2007)</p>
 +
 
 +
<p>Genetic Suppression of a dnaG Mutation in Escherichia coli Katayama (1989)</p>
 +
 
 +
<p>Genome-wide expression profiling in Escherichia coli K-12 Richmond (1999)</p>
 +
 
 +
<p>Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays Bernstein (2002)</p>
 +
 
 +
<p>The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds Ang (1989)</p>
 +
 
 +
<p>Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110 Hayashi (2006)</p>
 +
 
 +
<p>Idempotent Vector Design for Standard Assembly of Biobricks Knight 2003</p>
 +
 
 +
<p>Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli Morgan (1977)</p>
 +
 
 +
<p>Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization Meyer (2000)</p>
 +
 
 +
<p>In vivo transposition of mariner based elements in enteric bacteria and mycobacteria Rubin (1999)</p>
 +
 
 +
<p>Influences on gene expression in vivo by a Shine-Dalgarno sequence Jin (2006)</p>
 +
 
 +
<p>Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome Posfai (1999)</p>
 +
 
 +
<p>Measuring the activity of BioBrick promoters using an in vivo reference standard Kelly (2009)</p>
 +
 
 +
<p>One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome Gibson (2008)</p>
 +
 
 +
<p>One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Datsenko (2000)</p>
 +
 
 +
<p>Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome Masai (1988)</p>
 +
 
 +
<p>Phage integrases: biology and applications Groth (2004)</p>
 +
 
 +
<p>Primary structure of the essential replicon of the plasmid pSC101 Vocke (1983)</p>
 +
 
 +
<p>Programming gene expression with combinatorial promoters Cox (2007)</p>
 +
 
 +
<p>Rapid and efficient construction of markerless deletions in the Escherichia coli genome Yu (2008)</p>
 +
 
 +
<p>Rebuilding microbial genomes Holt (2007)</p>
 +
 
 +
<p>Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli DeVito (2007)</p>
 +
 
 +
<p>Refactoring bacteriophage T7 Chan (2005)</p>
 +
 
 +
<p>Refinement and standardization of synthetic biological parts and devices Canton (2008)</p>
 +
 
 +
<p>Regulation of the Synthesis of Ribonucleoside Diphosphate Reductase in Escherichia coli: Specific Activity of the Enzyme in Relationship to Perturbations of DNA Replication Filpula (1978)</p>
 +
 
 +
<p>Regulon DB online database</p>
 +
 
 +
<p>Replication of Escherichia coli requires DNA polymerase 1 Yamamoto (1974)</p>
 +
 
 +
<p>Replication of plasmid pSC101 Manen (1991)</p>
 +
 
 +
<p>RNA processing in prokaryotic cells Apirion (1993)</p>
 +
 
 +
<p>Replication-related organization of bacterial genomes, The Rocha (2004)</p>
 +
 
 +
<p>Ribosome biogenesis and the translation process in Escherichia coli Kaczanowska (2007)</p>
 +
 
 +
<p>Scarless engineering of the Escherichia coli genome Feher (2008)</p>
 +
 
 +
<p>Setting the standard in synthetic biology Arkin (2008)</p>
 +
 
 +
<p>Shear breakage of DNA Dancis (1978)</p>
 +
 
 +
<p>Shear degradation of DNA Adam (1977)</p>
 +
 
 +
<p>Shear induced assembly of lambda phage DNA Haber (2000)</p>
 +
 
 +
<p>Shear-induced degradation of plasmid DNA Lengsfeld (2002)</p>
 +
 
 +
<p>Shear unzipping of DNA Chakrabarti (2009)</p>
 +
 
 +
<p>Site-directed Mutational Analysis for the ATP Binding of DnaA Protein Mizushima (1998)</p>
 +
 
 +
<p>Simple and highly efficient BAC recombineering using galK selection Warming (2005)</p>
 +
 
 +
<p>Spatial and temporal organization of replicating Escherichia coli chromosomes Lau (2003)</p>
 +
 
 +
<p>Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions Jendrejack (2002)</p>
 +
 
 +
<p>Structural basis for uracil recognition by archaeal family B DNA polymerases Fogg (2002)</p>
 +
 
 +
<p>Synthesis of Unmethylated Deoxyribonucleic Acid in a dnaB Temperature-Sensitive Mutant of Escherichia coli Starved for Methionine Bouch (1973)</p>
 +
 
 +
<p>Techniques: recombinogenic engineering - new options for cloning and manipulating DNA Muyrers (2001)</p>
 +
 
 +
<p>Transcription of foreign DNA in Escherichia coli Warren (2008)</p>
 +
 
 +
<p>Transformation of Escherichia coli with large DNA molecules by electroporation Sheng (1995)</p>
 +
 
 +
<p>Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes Dekhtyar (2008)</p>
 +
 
 +
<p>USERTM friendly DNA engineering and cloning method by uracil excision Bitinaite (2007)</p>
 +
 
 +
<p>Vector NTI, a balanced all-in-one sequence analysis suite Lu (2004) </p>
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> -->
<!-- <div align="justify" style="padding-left:20px; padding-right:20px"> -->
<div align="justify">
<div align="justify">
-
<font size="2"><P>The minimal <i>E. coli</i> genome has been a holy grail of biology for a number of years. <i>E. coli</i> is the most widely used cellular research tool by the molecular biology community. Since scientific research is based upon reductionism and simplification for understanding, a simplified version of an experimental model organism such as <i>E. coli</i> is, in principle, preferred as a chassis for experimentation. To reduce the <i>E. coli</i> genome to roughly 10% its original size shows a great simplification of this model organism.</P>
+
 
-
<P>
+
 
-
To create such an organism, we plan on building an artificial <i>E. coli</i> chromosome using the BioBytes chromosome assembly system and inserting it into living <i>E. coli</i>. We then intend to remove the host chromosome by making it incapable of division. This allows only the artificial, inserted chromosome to propagate through multiple generations as the cells grow and divide. This is markedly different than the current, time-consuming method of knocking out inessential genes, one at a time, in an effort to produce the minimal genome. It is this difference that we hope to exploit in our attempt to win the race to produce the minimal <i>E. coli</i> genome.</p>
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<table width="133" border="0" cellspacing="0" cellpadding="3"><tr><td align="center"><a href="http://www.website-hit-counters.com" target="_blank"><img src="http://www.website-hit-counters.com/cgi-bin/image.pl?URL=232857-4018" alt="" border="0" ></a></td></tr><tr><td align="center"><font style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #330006; text-decoration: none;">Get a free <a href="http://www.website-hit-counters.com" target="_blank" style="font-family: Geneva, Arial, Helvetica, sans-serif; font-size: 9px; color: #555556; text-decoration: none;" title="html hit counter">html hit counter</a> here.</font></td></tr></table>
 
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Latest revision as of 20:29, 9 October 2009

University of Alberta - BioBytes










































































































References

NEED TO PROPERLY FORMAT REFERENCES

A family of LIC vectors for high through-put cloning and purification of proteins Eschenfeldt (2009)

An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda red Fukiya (2004)

The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame Flower (1986)

Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation Lu (2006)

Binding of biotinylated DNA to streptavidin-coated polystyrene latex: effects of chain length and particle size Huang (1996)

Biochemistry of homologous recombination in Escherichia coli Kowalczykowski (1994)

BioWarehouse: a bioinformatics database warehouse toolkit Lee (2006)

Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome Hashimoto (2005)

Chromosomal Locations of the Genes for rRNA in Escherichia coli K-12t Ellwood (1982)

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector Shizuya (1992)

Coding-sequence determinants of gene expression in Escherichia coli Kudla (2009)

Compilation and analysis of Escherichia coli promoter DNA sequences Hawley (1983)

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium Gibson (2008)

Complete set of ORF clones of Escherichia coli ASKA library (a complete set of Escherichia coli K-12 ORF archive): unique resources for biological research Kitagawa (2005)

Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria Haldimann (2001)

Conjugal Mobilization of Plasmid DNA: Termination Frequency at the Origin of Transfer of Plasmid R1162 Rao (1994)

Control of rRNA Synthesis in Escherichia coli: a Systems Biology Approach Dennis (2004)

Construction of consecutive deletions of the Escherichia coli chromosome Kato (2007)

Construction of Escherichia coli K-12 in-frame, single gene knockout mutants: the Keio collection Baba (2006)

Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome Kato (2008)

Determination of the core of a minimal bacterial gene set Gil (2004)

DNA cloning by homologous recombination in Escherichia coli Zhang (2000)

DNA Replication Kornberg (1988)

DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine Kelman (1995)

Dynamic strength of molecular adhesion bonds Evans (1997)

Effect of hydrodynamic shear of DNA from T2 and T4 bacteriophages, The Davison (1959)

Effect of dna Mutations on the Replication of Plasmid pSC101 in Escherichia coli K-12 Hasunuma (1979)

Effect of shear on plasmid DNA in solution Levy (1999)

Emergent properties of reduced genome Escherichia coli Posfai (2006)

Engineering a reduced Escherichia coli genome Kolisnychenko (2002)

Engineering BioBrick vectors from BioBrick parts Shetty (2008)

Engineering of bacterial ribosomes: Replacement of all seven Escherichia coli rRNA operons by a single plasmid-encoded operon Nomura (1999)

Escherichia coli and its chromosome Reyes-Lamothe (unknown)

Escherichia coli physiology in Luria-Bertani broth Sezonov (2007)

Experimental determination and systems level analysis of essential genes in Escherichia coli MG1665 Gerdes (2003)

Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson (2009)

Fermentation pathways of Escherichia coli, The Clark (1989)

Folding and unfolding single RNA molecules under tension Woodside (2008)

Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations Siegele (1997)

Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli Herring (2007)

Gene splicing and mutagenesis by PCR driven overlap extension Heckman (2007)

Genetic Suppression of a dnaG Mutation in Escherichia coli Katayama (1989)

Genome-wide expression profiling in Escherichia coli K-12 Richmond (1999)

Global analysis of mRNA decay and abundance in Escherichia coli at single-gene resolution using two-color fluorescent DNA microarrays Bernstein (2002)

The Heat-Shock-Regulated grpE Gene of Escherichia coli Is Required for Bacterial Growth at All Temperatures but Is Dispensable in Certain Mutant Backgrounds Ang (1989)

Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110 Hayashi (2006)

Idempotent Vector Design for Standard Assembly of Biobricks Knight 2003

Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli Morgan (1977)

Identification of the mob Genes of Plasmid pSC101 and Characterization of a Hybrid pSC101-R1162 System for Conjugal Mobilization Meyer (2000)

In vivo transposition of mariner based elements in enteric bacteria and mycobacteria Rubin (1999)

Influences on gene expression in vivo by a Shine-Dalgarno sequence Jin (2006)

Markerless gene replacement in Escherichia coli stimulated by double strand break in the chromosome Posfai (1999)

Measuring the activity of BioBrick promoters using an in vivo reference standard Kelly (2009)

One step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome Gibson (2008)

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Datsenko (2000)

Operon Structure of dnaT and dnaC Genes Essential for Normal and Stable DNA Replication of Escherichia coli Chromosome Masai (1988)

Phage integrases: biology and applications Groth (2004)

Primary structure of the essential replicon of the plasmid pSC101 Vocke (1983)

Programming gene expression with combinatorial promoters Cox (2007)

Rapid and efficient construction of markerless deletions in the Escherichia coli genome Yu (2008)

Rebuilding microbial genomes Holt (2007)

Recombineering with tolC as a selectable/counter-selectable marker: remodeling the rRNA operons in Escherichia coli DeVito (2007)

Refactoring bacteriophage T7 Chan (2005)

Refinement and standardization of synthetic biological parts and devices Canton (2008)

Regulation of the Synthesis of Ribonucleoside Diphosphate Reductase in Escherichia coli: Specific Activity of the Enzyme in Relationship to Perturbations of DNA Replication Filpula (1978)

Regulon DB online database

Replication of Escherichia coli requires DNA polymerase 1 Yamamoto (1974)

Replication of plasmid pSC101 Manen (1991)

RNA processing in prokaryotic cells Apirion (1993)

Replication-related organization of bacterial genomes, The Rocha (2004)

Ribosome biogenesis and the translation process in Escherichia coli Kaczanowska (2007)

Scarless engineering of the Escherichia coli genome Feher (2008)

Setting the standard in synthetic biology Arkin (2008)

Shear breakage of DNA Dancis (1978)

Shear degradation of DNA Adam (1977)

Shear induced assembly of lambda phage DNA Haber (2000)

Shear-induced degradation of plasmid DNA Lengsfeld (2002)

Shear unzipping of DNA Chakrabarti (2009)

Site-directed Mutational Analysis for the ATP Binding of DnaA Protein Mizushima (1998)

Simple and highly efficient BAC recombineering using galK selection Warming (2005)

Spatial and temporal organization of replicating Escherichia coli chromosomes Lau (2003)

Stochastic simulations of DNA in flow: dynamics and the effects of hydrodynamic interactions Jendrejack (2002)

Structural basis for uracil recognition by archaeal family B DNA polymerases Fogg (2002)

Synthesis of Unmethylated Deoxyribonucleic Acid in a dnaB Temperature-Sensitive Mutant of Escherichia coli Starved for Methionine Bouch (1973)

Techniques: recombinogenic engineering - new options for cloning and manipulating DNA Muyrers (2001)

Transcription of foreign DNA in Escherichia coli Warren (2008)

Transformation of Escherichia coli with large DNA molecules by electroporation Sheng (1995)

Triad pattern algorithms for predicting strong promoter candidates in bacterial genomes Dekhtyar (2008)

USERTM friendly DNA engineering and cloning method by uracil excision Bitinaite (2007)

Vector NTI, a balanced all-in-one sequence analysis suite Lu (2004)