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Team:Utah State/Project
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| - | === | + | <b><i><a name="I">Introduction</a></b></i></font> |
| - | + | <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR> | |
| - | + | <p> <font face="Helvetica, Arial, San Serif" color =#000000>This is where we talk about more stuff</p> | |
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| - | + | <h2>Secretion Pathways</h2> | |
| + | <p>Secretion pathway information</p> | ||
| - | + | <h2>Universal Vectors</h2> | |
| + | <p>This chapter explains ba bla bla</p> | ||
Revision as of 22:39, 9 October 2009
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BioBricks without Borders:
Investigating a multi-host BioBrick vector and secretion of cellular products The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms. |
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Overall project
Project Details
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Introduction
Investigating a multi-host BioBrick vector and secretion of cellular products This is where we talk about more stuff |
Secretion Pathways
Secretion pathway information
Universal Vectors
This chapter explains ba bla bla
