Team:Utah State/Parts

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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]]
 
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]]
 
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="#ffffff">The Project</font>]]
 
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#A9A9A9">Parts</font>]]
 
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Notebook|<font color="#ffffff">Notebook</font>]]
 
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Protocols|<font color="#ffffff">Protocols</font>]]
 
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        <td width="172" id="ana"><span class="currentPage"><font size = 4>HOME</font></span><a href="#LaLa">Introduction</a><br />
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        <a href="www.google.com">Experiments</a><br />
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        <a href="www.google.com">Results</a>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Team"><font size = 4>TEAM</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Project"><font size = 4>PROJECT</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Parts"><font size = 4>PARTS</font></a></td>
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        <td id="nav"><a href="https://2009.igem.org/Team:Utah_State/Links"><font size = 4>LINKS</font></a></td>
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              <b><i>BioBricks without Borders:</b></i></font>
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              <p><font face="Helvetica, Arial, San Serif" color =green>Investigating a multi-host BioBrick vector and secretion of cellular products</font></p><HR>
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<p> <font size="2.5" face=" Tahoma, Helvetica, Arial" color =#000000>The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like <i>Pseudomonas putida</i>, <i>Rhodobacter sphaeroides</i>, and <i>Synechocystis</i> PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation.  Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense.  To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions.  To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.</p>
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            <p>  <font size="4" face="Helvetica, Arial, San Serif" color =green>OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED.  PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!</font></p>
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
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Revision as of 04:00, 10 October 2009

USU iGem Untitled Document

HOMEIntroduction
Experiments
Results
BioBricks without Borders:

Investigating a multi-host BioBrick vector and secretion of cellular products

The aim of the Utah State University iGEM project is to develop improved upstream and downstream processing strategies for manufacturing cellular products using the standardized BioBrick system. First, we altered the broad-host range vector pRL1383a to comply with BioBrick standards and enable use of BioBrick constructs in organisms like Pseudomonas putida, Rhodobacter sphaeroides, and Synechocystis PCC6803. This vector will facilitate exploitation of advantageous characteristics of these organisms, such as photosynthetic carbon assimilation. Following expression, product recovery poses a difficult and expensive challenge. Downstream processing of cellular compounds, like polyhydroxyalkanoates (PHAs), commonly represents more than half of the total production expense. To counter this problem, secretion-promoting BioBrick devices were constructed through genetic fusion of signal peptides with protein-coding regions. To demonstrate this, the secretion of PHA granule-associated proteins and their affinity to PHA was investigated. Project success will facilitate expression and recovery of BioBrick-coded products in multiple organisms.

OUR SITE IS STILL UNDER CONSTRUCTION AND OUR INFORMATION IS BEING ADDED. PLEASE COME BACK IN A FEW WEEKS TO SEE OUR PROJECT!


This is where more text can go


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