Team:UCSF/Notebook
From 2009.igem.org
(Difference between revisions)
(→Dictyostelium Group) |
(→Dictyostelium Group) |
||
Line 13: | Line 13: | ||
*1. Design brakes, accelerators, steering wheels for Dictyostelium by modulating Pip3 in the cell. | *1. Design brakes, accelerators, steering wheels for Dictyostelium by modulating Pip3 in the cell. | ||
*2. PCR new parts including catalytic and localization domains | *2. PCR new parts including catalytic and localization domains | ||
- | *3. Create combinations of localization domains and catalytic domains | + | *3. Create combinations of localization domains and catalytic domains |
*4. Analyze data. Create histograms and compare if our plasmid had any effect to dicty. Ex. cause it to move faster or slower. | *4. Analyze data. Create histograms and compare if our plasmid had any effect to dicty. Ex. cause it to move faster or slower. | ||
Revision as of 08:09, 11 October 2009
HL60 Group
- Main Goals:
- 1. GPCR Screening
- 2.Forced localization of effector proteins to GPCRs
>
Dictyostelium Group
- Main Goals:
- 1. Design brakes, accelerators, steering wheels for Dictyostelium by modulating Pip3 in the cell.
- 2. PCR new parts including catalytic and localization domains
- 3. Create combinations of localization domains and catalytic domains
- 4. Analyze data. Create histograms and compare if our plasmid had any effect to dicty. Ex. cause it to move faster or slower.
>
- Allen Cai's notebook:
- Alex Smith's notebook:
- Edna Miao's notebook:
- Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with pTen mutated dicty cells.
- Ryan Quan's notebook: