August/16 August 2009

From 2009.igem.org

(Difference between revisions)
(→C4HSL P(tetR)+rhlI)
 
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  [[Image:Planning_osaka.PNG]]<br>
  [[Image:Planning_osaka.PNG]]<br>
-
=='''3O6HSL'''  P(tetR)+luxI<br>==
+
=='''3O<sub>6</sub>HSL'''  P(tetR)+luxI<br>==
1.RBS + luxI<br>
1.RBS + luxI<br>
Line 46: Line 46:
</table>
</table>
-
=='''C4HSL'''  P(tetR)+rhlI<br>==
+
=='''C<sub>4</sub>HSL'''  P(tetR)+rhlI<br>==
1.RBS + rhII
1.RBS + rhII
Line 108: Line 108:
</table>
</table>
-
=='''3OH,C14:1-HSL'''  P(tetR)+cinI<br>==
+
=='''3OH,C<sub>14</sub>:1-HSL'''  P(tetR)+cinI<br>==
1. RBS + cinI<br>
1. RBS + cinI<br>
Line 145: Line 145:
-
=='''3O6HSL'''  luxpL+luxR+luxpR==
+
=='''3O<sub>6</sub>HSL'''  luxpL+luxR+luxpR==
[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)<br>
[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)<br>
pSB1A2<br>
pSB1A2<br>
-
=='''C4HSL'''  p(tetR) + rhlR  +  RhlR+C4==
+
=='''C<sub>4</sub>HSL'''  p(tetR) + rhlR  +  RhlR+C<sub>4</sub>==
1.RhlR+p(Rhl)<br>
1.RhlR+p(Rhl)<br>
'''K204005'''
'''K204005'''
<table border="1" Frame="box">
<table border="1" Frame="box">
<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
-
<tr><td>RhlR</td><td>p(RhlR)</td></tr>
+
<tr><td>p(RhlR)</td><td>RhlR</td></tr>
-
<tr><td>[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]</td><td>[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]</td></tr>
+
<tr><td>[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]</td><td>[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]</td></tr>
-
<tr><td>4941bp</td><td>884bp</td></tr>
+
<tr><td>4476bp</td><td>884bp</td></tr>
<tr><td>pSB2K3</td><td></td></tr>
<tr><td>pSB2K3</td><td></td></tr>
</table>
</table>
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</table>
</table>
-
=='''3OH,C14:1-HSL'''  p(tetR) + cinR + cinR==
+
=='''3OH,C<sub>14</sub>:1-HSL'''  p(tetR) + cinR + cinR==
1. RBS + cinR<br>
1. RBS + cinR<br>
'''K204003'''
'''K204003'''
Line 251: Line 251:
<tr><td>pSB1A2</td><td></td></tr>
<tr><td>pSB1A2</td><td></td></tr>
</table>
</table>
 +
 +
 +
[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]

Latest revision as of 07:54, 12 October 2009

parts planning

teble sample

VectorInsert
namename
link to BBa~link to BBa~
total size (bp)insert size (bp)
Plasmid


Contents

Signal generator

signal generator
 
Planning osaka.PNG

3O6HSL P(tetR)+luxI

1.RBS + luxI

Vector(S+P)Insert(X+P)
RBSluxI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
2091bp585bp
pSB1A2


2.RBS+ luxI + terminator

Vector(E+X)Insert(E+S)
double terminatorRBS+luxI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp579bp
pSB1AK3


3.P(tetR)+RBS+ luxI + terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS+luxI+terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp726bp
pSB1A2

C4HSL P(tetR)+rhlI

1.RBS + rhII

Vector(S+P)Insert(X+P)
RBSrhII
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/Part:BBa_C0170 BBa_C0170]
2091bp609bp
pSB1A2


2.RBS + rhII + terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + rhII
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp621bp
pSB1AK3


3.P(tetR) + RBS + rhlI + terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + rhII +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp750bp
pSB1A2

AI-1 P(tetR)+lasI

1.RBS + LasI

Vector(S+P)Insert(X+P)
RBSLasI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078]
2091bp609bp
pSB1A2


2.RBS + LasI +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + LasI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp654bp
pSB1AK3


3.P(tetR) + RBS + LasI +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2091bp609bp
pSB1A2

3OH,C14:1-HSL P(tetR)+cinI

1. RBS + cinI
K204006

Vector(S+P)Insert(X+P)
RBScinI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076]
2091bp702bp
pSB1A2


2.RBS + cinI +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + cinI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp714bp
pSB1AK3


3.P(tetR) + RBS + cinI +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp843bp
pSB1A2

receiver

signal receiver
 
Planning osaka receiver.PNG


3O6HSL luxpL+luxR+luxpR

[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2


C4HSL p(tetR) + rhlR + RhlR+C4

1.RhlR+p(Rhl)
K204005

Vector(E+X)Insert(E+S)
p(RhlR)RhlR
[http://partsregistry.org/Part:BBa_I14017 BBa_I14017][http://partsregistry.org/Part:BBa_I1466 BBa_I1466]
4476bp884bp
pSB2K3

2.p(tetR)+RhlR+p(Rhl)

Vector(S+P)Insert(X+P)
p(tetR)RhlR+p(Rhl)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp935bp
pSB1A2

AI-1 p(tetR) + lasR + LasR+pAI

1. RBS + lasR
K204001,K204004

Vector(S+P)Insert(X+P)
RBSlasR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 BBa_C0179]
2091bp723bp
pSB1A2


2.RBS + lasR +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + lasR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp735bp
pSB1AK3


3.P(tetR) + RBS + lasR +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp864bp
pSB1A2


4.P(tetR) + RBS + lasR +terminator +lasR+pAI

Vector(E+X)Insert(E+S)
lasR+pAIP(tetR) + RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
2243bp918bp
pSB1A2

3OH,C14:1-HSL p(tetR) + cinR + cinR

1. RBS + cinR
K204003

Vector(S+P)Insert(X+P)
RBScinR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077]
2091bp762bp
pSB1A2


2.RBS + cinR +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + cinR
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
3189bp774bp
pSB1AK3


3.P(tetR) + RBS + cinR +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp903bp
pSB1A2


4.P(tetR) + RBS + cinR +terminator +p(cinR)

Vector(E+X)Insert(E+S)
p(cinR)P(tetR) + RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
2304bp957bp
pSB1A2


back to NOTES

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