"
Team:UCSF/Notebook
From 2009.igem.org
(Difference between revisions)
(→Dictyostelium Group) |
(→Dictyostelium Group) |
||
| Line 14: | Line 14: | ||
== '''Dictyostelium Group''' == | == '''Dictyostelium Group''' == | ||
* '''Main Goals:''' | * '''Main Goals:''' | ||
| - | ''Engineering brakes and accelerators:'' | + | * ''Engineering brakes and accelerators:'' |
* ''modulating PIP3 polarity by building synthetic protein based feedback loops'' | * ''modulating PIP3 polarity by building synthetic protein based feedback loops'' | ||
| - | workflow: | + | * workflow: |
*1. PCR parts: catalytic and localization domains | *1. PCR parts: catalytic and localization domains | ||
*2. Create combinations of localization domains and catalytic domains | *2. Create combinations of localization domains and catalytic domains | ||
Revision as of 17:03, 12 October 2009
HL60 Group
- Main Goals:
- Engineering Navigation:
- sending cells to new targets - GPCR Screening
- tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
>
Dictyostelium Group
- Main Goals:
- Engineering brakes and accelerators:
- modulating PIP3 polarity by building synthetic protein based feedback loops
- workflow:
- 1. PCR parts: catalytic and localization domains
- 2. Create combinations of localization domains and catalytic domains
- 3. Generate Dictyostelium strains expressing feedback elements
- 4. Measure motility parameters (speed, directionality)
- 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?
>
- Allen Cai's notebook:
- Alex Smith's notebook:
- Edna Miao's notebook: Transformed new constructs into Dicty, analyzed data, and worked with PTEN null dicty.
- Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with pTen mutated dicty cells.
- Ryan Quan's notebook:
