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Team:UNIPV-Pavia/Methods Materials/Absorbance
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== <html><font class="dayw_style">Absorbance and water dispensation</font></html> == | == <html><font class="dayw_style">Absorbance and water dispensation</font></html> == | ||
| - | We find no relevant differences between the measures before and after the dispensation of different volumes of water in the well, both for bacterial coltures and simple liquid | + | We find no relevant differences between the measures before and after the dispensation of different volumes of water in the well, both for bacterial coltures and simple liquid growth medium. |
You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9, 07/07/09. | You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9, 07/07/09. | ||
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[[Image:unipv_asb_volume.jpg| 500px|Absorbance and volume|thumb|center|Absorbance and volume]] | [[Image:unipv_asb_volume.jpg| 500px|Absorbance and volume|thumb|center|Absorbance and volume]] | ||
| - | == <html><font class="dayw_style">Absorbance and dilutions in liquid | + | == <html><font class="dayw_style">Absorbance and dilutions in liquid growth medium</font></html> == |
The proportionality between absorbance measurement (OD600) and colture's dilutions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well, regardless of the total volume present in the well. | The proportionality between absorbance measurement (OD600) and colture's dilutions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well, regardless of the total volume present in the well. | ||
| - | This experiment was done in two different | + | This experiment was done in two different growth mediums, LB and M9, and in three different total volumes in the well, 100μl, 200μl and 300μl. |
By fitting a linear regression model on the experimental data we estimate a coefficient of proportionality of 0.0018 for LB medium and 0.0035 of for M9. | By fitting a linear regression model on the experimental data we estimate a coefficient of proportionality of 0.0018 for LB medium and 0.0035 of for M9. | ||
Revision as of 22:10, 12 October 2009
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Measurements - Absorbance |
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Absorbance and water dispensationWe find no relevant differences between the measures before and after the dispensation of different volumes of water in the well, both for bacterial coltures and simple liquid growth medium. You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9, 07/07/09.
Absorbance and volumeThe proportionality between absorbance (OD600) measurement and volume of colture in the well was verified. By fitting a linear regression model on the experimental data we estimate a coefficient of proportionality of 8.27e-4. You can find more informations about the experiment at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.9bis, 10/10/09.
 Absorbance and dilutions in liquid growth mediumThe proportionality between absorbance measurement (OD600) and colture's dilutions was verified, confirming the possibility of using OD600 to measure the bacterial quantity in the well, regardless of the total volume present in the well. This experiment was done in two different growth mediums, LB and M9, and in three different total volumes in the well, 100μl, 200μl and 300μl. By fitting a linear regression model on the experimental data we estimate a coefficient of proportionality of 0.0018 for LB medium and 0.0035 of for M9. You can find more informations about the experiments at [http://aimed11.unipv.it/iGEM2009 Download Protocol] - Test n.20, 13/08/09 and Test n.20 "M9", 28/08/09.
 
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