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Team:UCSF/Notebook
From 2009.igem.org
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(→Dictyostelium Group) |
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*[[Allen Cai's notebook]]: | *[[Allen Cai's notebook]]: | ||
*[[Alex Smith's notebook]]: | *[[Alex Smith's notebook]]: | ||
| - | *[[Edna Miao's notebook]]: | + | *[[Edna Miao's notebook]]: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty. |
*[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. | *[[Ethan Chan's notebook]]: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells. | ||
*[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them. | *[[Ryan Quan's notebook]]:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them. | ||
Revision as of 03:52, 13 October 2009
HL60 Group
- Goals:
- Engineering Navigation:
- sending cells to new targets - GPCR Screening
- tuning receptor sensitivity - Forced localization of effector proteins to GPCRs
- Payload:
- can we make our cells carry stuff? - attach beads to cells
>
- Cathy Liu's notebook:
- Eric Wong's notebook:
- Jackie Tam's notebook:
- Ryan Liang's notebook:I cloned parts to be used in the GPCR screens, transfected HL-60 cells with various different GPCRs, ran transwell assays, and analyzed/compared transwell assay results.
Dictyostelium Group
- Goals:
- Engineering brakes and accelerators:
- modulating PIP3 polarity by building synthetic protein based feedback loops
- workflow:
- 1. PCR parts: catalytic and localization domains
- 2. Create combinations of localization domains and catalytic domains
- 3. Generate Dictyostelium strains expressing feedback elements
- 4. Measure motility parameters (speed, directionality)
- 3. Analyze data. Create histograms and compare if our plasmid had any effect to dicty.Do they cause them to move faster or slower?
>
- Allen Cai's notebook:
- Alex Smith's notebook:
- Edna Miao's notebook: I transformed new constructs into Dicty, analyzed data, and worked with wildtype and PTEN null dicty.
- Ethan Chan's notebook: Constructed Dicty cells with new parts, analyzed data, and worked with wildtype and PTEN null dicty cells.
- Ryan Quan's notebook:I worked on team dicty and taught the students some basic cloning techniques but mainly learned many new things along with them.
