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Team:IPN-UNAM-Mexico/Notebook/August 10 2009
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| + | <td style="width:553px; height:51px "><img src="https://static.igem.org/mediawiki/2009/7/7f/México44MX.png" alt="" width="45" height="45" hspace="15"><a href="http://www.unam.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/8/8a/UNAMMX.gif" alt="UNAM" width="50" height="56" hspace="50"></a></a><a href="http://www.fciencias.unam.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/6/6e/FCienciasMX.png" alt="FCIENCIAS" width="50" height="57" hspace="50"></a><a href="http://www.ira.cinvestav.mx/" class="style7"><img src="https://static.igem.org/mediawiki/2009/c/ce/CinvestavMX.gif" alt="CINVESTAV" width="50" height="62" hspace="50"></a></a></td> | ||
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| + | <table width="100%" height=100%" "border="0" cellspacing="0" cellpadding="0"> | ||
| + | <tr> | ||
| + | <td><p align="right"> </p> | ||
| + | <p align="right"><img src="https://static.igem.org/mediawiki/2009/a/a6/LabIconMX.png" alt="LabNotebook" width="30" height="35"> <span><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook">Back to Notebook</a></span></p> | ||
| + | <p><strong>August 10th, 2009</strong></p> | ||
| + | <p>Calculus for Mix; plasmid linearization.</p> | ||
| + | <p>For 20 reactions.</p> | ||
| + | <p align="left" class="style8"> </p> | ||
| + | <table width="24%" border="0" align="left" cellpadding="0" cellspacing="0"> | ||
| + | <tr> | ||
| + | <td> </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table width="54%" border="1" align="left" cellpadding="0" cellspacing="0"> | ||
| + | |||
| + | <tr> | ||
| + | <td class="style8"><div align="center" class="style11">Mix</div></td> | ||
| + | <td class="style11"><div align="center" class="style11">For every Eppendorf tube</div></td> | ||
| + | <td class="style11"><div align="center" class="style11">For 20 reactions</div></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="20%" valign="top" class="style8"><p align="center" class="style8">PlasmidicDNA</p></td> | ||
| + | <td width="51%" valign="top" class="style8"><p align="center" class="style8">3 μl</p></td> | ||
| + | <td width="29%" valign="top" class="style8"><p align="center" class="style8">60 μl</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">Buffer 2</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">1 μl</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">20 μl</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">BSA</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">0.5μl</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">10 μl</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">EcoRI</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">0.5 μl / 5 μll</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">10 μl / 100 μl</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">H<span class="style12">2</span>O</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">5 μl / 10 μl vf</p></td> | ||
| + | <td valign="top" class="style8"><p align="center" class="style8">100 μl / 200 μl vf</p></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table width="147" height="97" border="0"> | ||
| + | <tr> | ||
| + | <td height="75"> </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p>Electrophoresis gel load:</p> | ||
| + | <ul> | ||
| + | <li>3 μl of colorant</li> | ||
| + | <li>10 μl of digestion</li> | ||
| + | <li>3 μl of molecular weight marker</li> | ||
| + | </ul> | ||
| + | <p> </p> | ||
| + | <p>Load order.</p> | ||
| + | <p>Well Plasmid Well Plasmid</p> | ||
| + | <p>2 16,2” 13 3.3<br> | ||
| + | 3 4:23 L2 14 22.1<br> | ||
| + | 4 15:3 L2 15 19.2*<br> | ||
| + | 5 18.2* 16 24.2”<br> | ||
| + | 6 2.3</p> | ||
| + | <p>Well Well<br> | ||
| + | 1 1kb 2 μl 12 2.3<br> | ||
| + | 2 16.2” 13 14.2<br> | ||
| + | 3 4:23 L2 14 3.3<br> | ||
| + | 4 15:3 L2 15 22.1<br> | ||
| + | 5 18.2 15 19.1*<br> | ||
| + | 6 15.13</p> | ||
| + | <p align="left"> </p> | ||
| + | <p>Horizontal Gene Transfer (HGT).<br> | ||
| + | Acording to the model proposed to Arturo, we made a search for the candidate bioparts to make their elusion and transformation, which got in the next way:</p> | ||
| + | <p>2 Constructions:</p> | ||
| + | <p><br> | ||
| + | Construction 1:<br> | ||
| + | Biopart BBa_K145201</p> | ||
| + | <p>J23116 + B0034 + C0040 + B0010 + B0012</p> | ||
| + | <p>Construction 2:<br> | ||
| + | The first option for the construction 2 is made up from two devices: BBa_J01101 and Bba_I763011. The second one consists in asking Boston for the construction BBa_I763027. We will tell Arturo about this posibility.<br> | ||
| + | First option:<br> | ||
| + | Device 1.<br> | ||
| + | B0040 + B0034 + C0051 + B0010 + B0012<br> | ||
| + | Device 2.<br> | ||
| + | B0051 + B0034 + J04031</p> | ||
| + | <p align="left"> </p> | ||
| + | <p align="left" class="style8">LOCATION</p> | ||
| + | <table width="379" border="1" cellpadding="0" cellspacing="0"> | ||
| + | <tr> | ||
| + | <td width="115" valign="top"><p> </p></td> | ||
| + | <td width="99" valign="top" ><p><strong>Plate</strong></p></td> | ||
| + | <td width="51" valign="top" ><p><strong>Well</strong></p></td> | ||
| + | <td width="104" valign="top" ><p><strong>Plasmid</strong></p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="115" valign="top"><p>Device 1 (piece1)</p></td> | ||
| + | <td width="99" valign="top" ><p>2009 kitplate1</p></td> | ||
| + | <td width="51" valign="top" ><p>11H</p></td> | ||
| + | <td width="104" valign="top" ><p>PSB1A2</p></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td width="115" valign="top"><p>Device 2 (piece 2)</p></td> | ||
| + | <td width="99" valign="top" ><p>2009 kitplate1</p></td> | ||
| + | <td width="51" valign="top" ><p>15L</p></td> | ||
| + | <td width="104" valign="top" ><p>PSB1A2</p></td> | ||
| + | </tr> | ||
| + | </table> | ||
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| + | <p align="left" > </p> | ||
| + | <p align="left" > </p> | ||
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Latest revision as of 08:18, 14 October 2009
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