Team:UNIPV-Pavia/Freezer

From 2009.igem.org

(Difference between revisions)
 
(13 intermediate revisions not shown)
Line 254: Line 254:
<td class="prova">sequence ok (MIT)</td>
<td class="prova">sequence ok (MIT)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A1</td>
+
  <tr class="in_freezer"><td class="prova">A1 (BBa_K173000)</td>
<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
<td class="prova">J23100(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                 <td class="prova">Jun 11</td>
                 <td class="prova">Jun 11</td>
Line 261: Line 261:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A2</td>
+
  <tr class="in_freezer"><td class="prova">A2 (BBa_K173001)</td>
<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
<td class="prova">J23101(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                 <td class="prova">Jun 11</td>
                 <td class="prova">Jun 11</td>
Line 268: Line 268:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a></td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A3-1</td>
+
  <tr class="in_freezer"><td class="prova">A3-1 (BBa_K173004)</td>
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
<td class="prova">I732017-B0015<br>(RBS-lacZ-TT)</td>
                 <td class="prova">Jun 12</td>
                 <td class="prova">Jun 12</td>
Line 275: Line 275:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A3_B0015.jpg">(digestion PstI)</a> sequence ok -long part- (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A4</td>
+
  <tr class="in_freezer"><td class="prova">A4 (BBa_K173005)</td>
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
<td class="prova">P0440-R0040<br>(RBS-tetR-TT-Ptet)</td>
                 <td class="prova">Jun 11</td>
                 <td class="prova">Jun 11</td>
Line 282: Line 282:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_colonypcr_A1_A2_A4.jpg">(PCR)</a> sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A5</td>
+
  <tr class="in_freezer"><td class="prova">A5 (BBa_K173006)</td>
<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td>
<td class="prova">A4-A3<br>(RBS-tetR-TT-Ptet-RBS-lacZ-TT)</td>
                 <td class="prova">Jun 19</td>
                 <td class="prova">Jun 19</td>
Line 289: Line 289:
<td class="prova">sequence ok -long part- (BMR)</td>
<td class="prova">sequence ok -long part- (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A6</td>
+
  <tr class="in_freezer"><td class="prova">A6 (BBa_K173007)</td>
<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td>
<td class="prova">J23100(Jun 4)-A4<br>(Pcon-RBS-tetR-TT-Ptet)</td>
                 <td class="prova">Jun 19</td>
                 <td class="prova">Jun 19</td>
Line 303: Line 303:
<td class="prova">sequence ok (BMR)</td>
<td class="prova">sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A7</td>
+
  <tr class="in_freezer"><td class="prova">A7 (BBa_K173002)</td>
<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
<td class="prova">J23118(Jun 4)-E0240<br>(Pcon-RBS-GFP-TT)</td>
                 <td class="prova">Jun 25</td>
                 <td class="prova">Jun 25</td>
Line 345: Line 345:
<td class="prova">sequence BAD (BMR)</td>
<td class="prova">sequence BAD (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A8pg</td>
+
  <tr class="in_freezer"><td class="prova">A8pg (BBa_K173008)</td>
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
<td class="prova">A4-E0240<br>(RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                 <td class="prova">Jul 3</td>
                 <td class="prova">Jul 3</td>
Line 352: Line 352:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_digestion_A8_A9_10pg.jpg">(digestion E-P)</a></td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A9pg</td>
+
  <tr class="in_freezer"><td class="prova">A9pg (BBa_K173009)</td>
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
<td class="prova">A6-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                 <td class="prova">Jul 3</td>
                 <td class="prova">Jul 3</td>
Line 387: Line 387:
<td class="prova">sequence BAD (MIT, BMR)</td>
<td class="prova">sequence BAD (MIT, BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A12-2</td>
+
  <tr class="in_freezer"><td class="prova">A12-2 (BBa_K173011)</td>
<td class="prova">J23118(Jun 4)-A4<br></td>
<td class="prova">J23118(Jun 4)-A4<br></td>
                 <td class="prova">Jul 16</td>
                 <td class="prova">Jul 16</td>
Line 408: Line 408:
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td>
<td class="prova">NON PURE PLASMID! <a href="https://2009.igem.org/Image:Pv_digestion_A14.jpg">(digestion)</a> and the A11 sample used for ligation was MUTATED (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A14pg<br>thrown away</td>
+
  <tr class="in_freezer"><td class="prova">A14pg <br>thrown away</td>
<td class="prova">A11-E0240<br></td>
<td class="prova">A11-E0240<br></td>
                 <td class="prova">Jul 16</td>
                 <td class="prova">Jul 16</td>
Line 418: Line 418:
<td class="prova">PnhaA-RBS-GFP-TT<br></td>
<td class="prova">PnhaA-RBS-GFP-TT<br></td>
                 <td class="prova">Jul 29</td>
                 <td class="prova">Jul 29</td>
-
<td class="prova">?</td>
+
<td class="prova">NEB 10-beta</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
<td class="prova">sequence WRONG! it is actually K116002!!!</td>
<td class="prova">sequence WRONG! it is actually K116002!!!</td>
Line 425: Line 425:
<td class="prova">PnhaA-RBS-GFP-TT<br></td>
<td class="prova">PnhaA-RBS-GFP-TT<br></td>
                 <td class="prova">Jul 29</td>
                 <td class="prova">Jul 29</td>
-
<td class="prova">?</td>
+
<td class="prova">NEB 10-beta</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
<td class="prova">sequence WRONG! it is actually J33204!!!</td>
<td class="prova">sequence WRONG! it is actually J33204!!!</td>
Line 478: Line 478:
<td class="prova">sequence ok (BMR)</td>
<td class="prova">sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">MRGENE1</td>
+
  <tr class="in_freezer"><td class="prova">MRGENE1 (BBa_K173016)</td>
<td class="prova">pdc</td>
<td class="prova">pdc</td>
                 <td class="prova">Jul 17</td>
                 <td class="prova">Jul 17</td>
Line 499: Line 499:
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">MRGENE2</td>
+
  <tr class="in_freezer"><td class="prova">MRGENE2 (BBa_K173017)</td>
<td class="prova">adhB</td>
<td class="prova">adhB</td>
                 <td class="prova">Jul 17</td>
                 <td class="prova">Jul 17</td>
Line 520: Line 520:
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
<td class="prova">antibiotic ok<br>sequence ok (Mr Gene)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B1-13</td>
+
  <tr class="in_freezer"><td class="prova">B1-13 (BBa_K173013)</td>
<td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td>
<td class="prova">B0030-MRGENE1<br>(RBS-pdc)</td>
                 <td class="prova">Jul 28</td>
                 <td class="prova">Jul 28</td>
Line 527: Line 527:
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td>
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion E-P</a><br>, <a href="https://2009.igem.org/Image:Pv_digestion_B1_S-P.jpg">digestion S-P</a> and <a href="https://2009.igem.org/Image:Pv_B1E-S_B2E-S_B0015E-X_cut.jpg">digestion E-S</a>)<br>sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B2-5</td>
+
  <tr class="in_freezer"><td class="prova">B2-5 (BBa_K173014)</td>
<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td>
<td class="prova">B0030-MRGENE2<br>(RBS-adhB)</td>
                 <td class="prova">Jul 23</td>
                 <td class="prova">Jul 23</td>
Line 534: Line 534:
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td>
<td class="prova">length ok (<a href="https://2009.igem.org/Image:Pv_B2pcr_A14pgPstI.jpg">PCR</a><br> and <a href="https://2009.igem.org/Image:Pv_digestion_B1_B2.jpg">digestion S-P</a>)<br>sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B3-5</td>
+
  <tr class="in_freezer"><td class="prova">B3-5 (BBa_K173018)</td>
<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td>
<td class="prova">B1-B0015<br>(RBS-pdc-TT)</td>
                 <td class="prova">Jul 31</td>
                 <td class="prova">Jul 31</td>
Line 541: Line 541:
<td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td>
<td class="prova">length ok+extra band (<a href="https://2009.igem.org/Image:Pv_B3_B4_digestion_PstI_XhoI.jpg">digestion XhoI-P</a><br> and <a href="https://2009.igem.org/Image:Pv_B3_digestion_X-P.jpg">digestion X-P</a>)<br>sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B4-2</td>
+
  <tr class="in_freezer"><td class="prova">B4-2 (BBa_K173019)</td>
<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td>
<td class="prova">B2-B0015<br>(RBS-adhB-TT)</td>
                 <td class="prova">Jul 31</td>
                 <td class="prova">Jul 31</td>
Line 555: Line 555:
<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td>
<td class="prova">length ok+2 extra bands <a href="https://2009.igem.org/Image:Pv_digestion_B5_B6_E-P.jpg">(digestion E-P)</a><br>sequence ok (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B6-3</td>
+
  <tr class="in_freezer"><td class="prova">B6-3 (BBa_K173020)</td>
<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td>
<td class="prova">B2-B3<br>(RBS-adhB-RBS-pdc-TT)</td>
                 <td class="prova">Aug 12</td>
                 <td class="prova">Aug 12</td>
Line 569: Line 569:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion S-P)</a> sequence MUTATED (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A11-3</td>
+
  <tr class="in_freezer"><td class="prova">A11-3 (BBa_K173010)</td>
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
<td class="prova">BOL1-R0011<br>(Pcon-RBS-lacI-TT-Plac)</td>
                 <td class="prova">Aug 13</td>
                 <td class="prova">Aug 13</td>
Line 590: Line 590:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(digestion)</a> sequence WRONG (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A15-3</td>
+
  <tr class="in_freezer"><td class="prova">A15-3 (BBa_K173015)</td>
<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
<td class="prova">F2620MIT1-K112808<br>(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)</td>
                 <td class="prova">Aug 14</td>
                 <td class="prova">Aug 14</td>
Line 611: Line 611:
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td>
<td class="prova">length ok <a href="https://2009.igem.org/Image:Pv_.jpg">(PCR)</a> sequence of A11-2(Aug13) was MUTATED (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A16-4</td>
+
  <tr class="in_freezer"><td class="prova">A16-4 (BBa_K173026)</td>
<td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
<td class="prova">A12-E0240<br>(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
                 <td class="prova">Aug 21</td>
                 <td class="prova">Aug 21</td>
Line 639: Line 639:
<td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td>
<td class="prova">sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B5new2-3</td>
+
  <tr class="in_freezer"><td class="prova">B5new2-3 (BBa_K173003)</td>
<td class="prova">B1-B4 (RBS-pdc-RBS-adhB-TT)</td>
<td class="prova">B1-B4 (RBS-pdc-RBS-adhB-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Sep 1</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1AK3</td>
<td class="prova">pSB1AK3</td>
<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B9-2</td>
+
  <tr class="in_freezer"><td class="prova">B9-2 (BBa_K173021)</td>
-
<td class="prova">F2620-B5 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
+
<td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Sep 4</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1AK3</td>
<td class="prova">pSB1AK3</td>
<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
<td class="prova">length ok (digestion E-P), sequence ok -long part- (BMR)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B10-2</td>
+
  <tr class="in_freezer"><td class="prova">B10-2 (BBa_K173022)</td>
<td class="prova">F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)</td>
<td class="prova">F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Sep 4</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1AK3</td>
<td class="prova">pSB1AK3</td>
Line 662: Line 662:
  <tr class="in_freezer"><td class="prova">B9new-1 to 5</td>
  <tr class="in_freezer"><td class="prova">B9new-1 to 5</td>
<td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
<td class="prova">F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 1</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1AK3</td>
<td class="prova">pSB1AK3</td>
Line 669: Line 669:
  <tr class="in_freezer"><td class="prova">A19</td>
  <tr class="in_freezer"><td class="prova">A19</td>
<td class="prova">F2620</td>
<td class="prova">F2620</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 1</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB4C5</td>
<td class="prova">pSB4C5</td>
Line 676: Line 676:
  <tr class="in_freezer"><td class="prova">A21</td>
  <tr class="in_freezer"><td class="prova">A21</td>
<td class="prova">A19-B5new2(-3)</td>
<td class="prova">A19-B5new2(-3)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 8</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB4C5</td>
<td class="prova">pSB4C5</td>
<td class="prova">length ok (digestion E-P)</td>
<td class="prova">length ok (digestion E-P)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A14S-3</td>
+
  <tr class="in_freezer"><td class="prova">A14S-3 (BBa_K173012)</td>
<td class="prova">J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)</td>
<td class="prova">J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240)</td>
                 <td class="prova">?</td>
                 <td class="prova">?</td>
Line 688: Line 688:
<td class="prova">length ok (digestion E-P)</td>
<td class="prova">length ok (digestion E-P)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">A17new</td>
+
  <tr class="in_freezer"><td class="prova">A17new (BBa_K173025)</td>
<td class="prova">Plac-RBS-GFP-TT (R0011-E0240)</td>
<td class="prova">Plac-RBS-GFP-TT (R0011-E0240)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Sep 4</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
Line 697: Line 697:
  <tr class="in_freezer"><td class="prova">A20</td>
  <tr class="in_freezer"><td class="prova">A20</td>
<td class="prova">F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
<td class="prova">F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 8</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
Line 711: Line 711:
  <tr class="in_freezer"><td class="prova">I714891 (3K3)</td>
  <tr class="in_freezer"><td class="prova">I714891 (3K3)</td>
<td class="prova">plasmid</td>
<td class="prova">plasmid</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Aug 21</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB3K3</td>
<td class="prova">pSB3K3</td>
<td class="prova">length ok (digestion E-P)</td>
<td class="prova">length ok (digestion E-P)</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B14</td>
+
  <tr class="in_freezer"><td class="prova">B14 (BBa_K173023)</td>
<td class="prova">F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)</td>
<td class="prova">F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 16</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>
<td class="prova">to screen</td>
<td class="prova">to screen</td>
             </tr>
             </tr>
-
  <tr class="in_freezer"><td class="prova">B15</td>
+
  <tr class="in_freezer"><td class="prova">B15 (BBa_K173024)</td>
<td class="prova">F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)</td>
<td class="prova">F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT)</td>
-
                 <td class="prova">?</td>
+
                 <td class="prova">Oct 16</td>
<td class="prova">TOP10</td>
<td class="prova">TOP10</td>
<td class="prova">pSB1A2</td>
<td class="prova">pSB1A2</td>

Latest revision as of 12:32, 21 October 2009

EthanolPVanimation.gif

Biobrick/
Ligation Name		 Description Creation Date Strain Vector Quality Control
J23100(J100)
replaced by "J23100 Jun4"		 constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23101(J101)
replaced by "J23101 Jun4"		 constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23102(J102) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23103(J103) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23104(J104) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23105(J105) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23106(J106) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23110(J110) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23112(J112) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23113(J113) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23114(J114) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23116(J116) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23117(J117) constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118)
replaced by "J23118 Jun4"		 constitutive promoter May 25 TOP10 J61002(Amp) sequence ok (MIT)
E0240(GFP3) GFP protein generator
(RBS-GFP-TT)		 May 25 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
K131009(E2) colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) May 28 TOP10 pSB1AK3 sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB) colE7 lysis gene May 28 TOP10 pSB1A2 sequence ok (MIT)
K124017(lysis21) Phage Lysis Cassette May 28 TOP10 pSB1AK3 sequence BAD (MIT)
B0015(TT) Double terminator May 28 TOP10 pSB1AK3 sequence ok (MIT)
I732017 (RBS-lacZ) RBS-lacZ May 28 TOP10 pSB1A2 long part (MIT)
B0030 RBS 0.6 Jun 4 TOP10 pSB1A2 sequence ok (MIT,BMR)
B0031 RBS 0.07 Jun 4 TOP10 pSB1A2 sequence ok (MIT)
B0032 RBS 0.3 Jun 4 TOP10 pSB1A2 sequence ok (MIT)
B0033 RBS 0.01 Jun 4 TOP10 pSB1A2 sequence ok (MIT)
B0034 RBS 1 Jun 4 TOP10 pSB1A2 sequence ok (MIT)
P0440(P04) RBS-tetR-TT Jun 4 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
R0040(Ptet) Ptet Jun 4 TOP10 pSB1A2 sequence ok (MIT)
T9002 Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT Jun 4 TOP10 pSB1A3 sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4) constitutive promoter Jun 4 TOP10 J61002(Amp) sequence ok (MIT,BMR)
J23101(J101 Jun4) constitutive promoter Jun 4 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118 Jun4) constitutive promoter Jun 4 TOP10 J61002(Amp) sequence ok (MIT)
A1 (BBa_K173000) J23100(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 TOP10 pSB1A2 length ok (PCR)
A2 (BBa_K173001) J23101(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 TOP10 pSB1A2 length ok (PCR)
A3-1 (BBa_K173004) I732017-B0015
(RBS-lacZ-TT)		 Jun 12 TOP10 pSB1AK3 length ok (digestion PstI) sequence ok -long part- (BMR)
A4 (BBa_K173005) P0440-R0040
(RBS-tetR-TT-Ptet)		 Jun 11 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
A5 (BBa_K173006) A4-A3
(RBS-tetR-TT-Ptet-RBS-lacZ-TT)		 Jun 19 TOP10 pSB1AK3 sequence ok -long part- (BMR)
A6 (BBa_K173007) J23100(Jun 4)-A4
(Pcon-RBS-tetR-TT-Ptet)		 Jun 19 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
BOL1 J23118-B0034-C0012-B0015
(Pcon-RBS-lacI-TT)		 Jun 23 TOP10 pSB1A2 sequence ok (BMR)
A7 (BBa_K173002) J23118(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 25 TOP10 pSB1A2 length ok (PCR)
R0011(Plac) Plac Jun 25 TOP10 pSB1A2 sequence ok (MIT)
K112808(ENTERO) holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) Jun 25 TOP10 pSB1A2 sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).
A8 A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 TOP10 pSB1A2 NON PURE PLASMID! (digestion E-P) but works
A9 A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 TOP10 pSB1A2 NON PURE PLASMID! (digestion E-P) but works
A10
thrown away		 K117000-B0015
(celB-TT)		 Jun 30 TOP10 pSB1AK3 sequence BAD (BMR)
A8pg (BBa_K173008) A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 TOP10 pSB1A2 length ok (digestion E-P)
A9pg (BBa_K173009) A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 TOP10 pSB1A2 length ok (digestion E-P)
A11-1
thrown away and replaced		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 TOP10 pSB1A2 length ok (PCR)
sequence MUTATED
A11-3
thrown away and replaced		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 TOP10 pSB1A2 length ok (PCR)
A11-6
thrown away and replaced		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 TOP10 pSB1A2 length ok (PCR)
F2620
thrown away		 Ptet-RBS-luxR-TT-Plux
 Jul 10 TOP10 pSB1A2 sequence BAD (MIT, BMR)
A12-2 (BBa_K173011) J23118(Jun 4)-A4
 Jul 16 TOP10 pSB1A2 length ok (PCR)
sequence ok (BMR)
A12-3
it is a backup of A12-2		 J23118(Jun 4)-A4
 Jul 16 TOP10 pSB1A2 length ok (PCR)
sequence ok (BMR)
A14-4
thrown away		 A11-E0240
 Jul 16 TOP10 pSB1A2 NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
A14pg
thrown away		 A11-E0240
 Jul 16 TOP10 pSB1A2 NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
K116001 (PnhaA) PnhaA-RBS-GFP-TT
 Jul 29 NEB 10-beta pSB1A2 sequence WRONG! it is actually K116002!!!
K116002 (PnhaA+GFP) PnhaA-RBS-GFP-TT
 Jul 29 NEB 10-beta pSB1A2 sequence WRONG! it is actually J33204!!!
K112405 (Pcad) cadAB promoter
 Jul 29 NEB 10-beta K112951(Amp+Cm) sequence ok (BMR)
P0412 RBS-lacI-TT
 Jul 29 DH5alpha pSB1A2 sequence ok (BMR)
I746902 GFPmut3+his tag generator under Pbad
 Jul 29 NEB 10-beta pSB1AK3 sequence ok -long part- (BMR)
I746903 GFPmut3+his tag generator under PT7
 Jul 29 NEB 10-beta pSB1AK3 sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same
K101017
to throw away!		 MioC promoter
 Jul 29 NEB 10-beta pSB1A2 sequence BAD (BMR)
F2620MIT1 Ptet-RBS-luxR-TT-Plux
 Jul 29 D1210(lacIq) pSB1A2 sequence ok (BMR)
F2620MIT2 Ptet-RBS-luxR-TT-Plux
 Jul 29 D1210(lacIq) pSB1A2 sequence ok (BMR)
MRGENE1 (BBa_K173016) pdc Jul 17 TOP10 pMK-RQ
(Kan)		 antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene)
MRGENE1-2 pdc Jul 17 TOP10 pMK-RQ
(Kan)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE1-3 pdc Jul 17 TOP10 pMK-RQ
(Kan)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE2 (BBa_K173017) adhB Jul 17 TOP10 pMA
(Amp)		 antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene)
MRGENE2-2 adhB Jul 17 TOP10 pMA
(Amp)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE2-3 adhB Jul 17 TOP10 pMA
(Amp)		 antibiotic ok
sequence ok (Mr Gene)
B1-13 (BBa_K173013) B0030-MRGENE1
(RBS-pdc)		 Jul 28 TOP10 pSB1A2 length ok (digestion E-P
, digestion S-P and digestion E-S)
sequence ok (BMR)
B2-5 (BBa_K173014) B0030-MRGENE2
(RBS-adhB)		 Jul 23 TOP10 pSB1A2 length ok (PCR
and digestion S-P)
sequence ok (BMR)
B3-5 (BBa_K173018) B1-B0015
(RBS-pdc-TT)		 Jul 31 TOP10 pSB1AK3 length ok+extra band (digestion XhoI-P
and digestion X-P)
sequence ok (BMR)
B4-2 (BBa_K173019) B2-B0015
(RBS-adhB-TT)		 Jul 31 TOP10 pSB1AK3 length ok (digestion XhoI-P)
sequence ok (BMR)
B5-3 B1-B4
(RBS-pdc-RBS-adhB-TT)		 Aug 12 TOP10 pSB1AK3 length ok+2 extra bands (digestion E-P)
sequence ok (BMR)
B6-3 (BBa_K173020) B2-B3
(RBS-adhB-RBS-pdc-TT)		 Aug 12 TOP10 pSB1AK3 length ok+2 extra bands (digestion E-P)
sequence ok (BMR)
A11-2
thrown away		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Aug 13 TOP10 pSB1A2 length ok (digestion S-P) sequence MUTATED (BMR)
A11-3 (BBa_K173010) BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Aug 13 TOP10 pSB1A2 length ok (digestion S-P) sequence ok (BMR)
A15-1
it is a backup copy of A15-3		 F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 TOP10 pSB1A2 length ok (digestion) sequence ok (BMR)
A15-2
thrown away		 F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 TOP10 pSB1A2 length ok (digestion) sequence WRONG (BMR)
A15-3 (BBa_K173015) F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 TOP10 pSB1A2 length ok (digestion) sequence ok (BMR)
B8-5
thrown away		 A4-B6
(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)		 Aug 21 TOP10 pSB1AK3 length ??? (digestion E-P) sequence WRONG (BMR)
A14-3
to throw away!		 A11(-2 Aug13)-E0240
(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)		 Aug 21 TOP10 pSB1A2 length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR)
A16-4 (BBa_K173026) A12-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Aug 21 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
A17-4
to throw away!		 R0011-E0240
(Plac-RBS-GFP-TT)		 Aug 28 TOP10 pSB1A2 length ok (PCR) sequence WRONG! ligation failed.
A18
thrown away		 A15(-2)-pSB3K3
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 28 TOP10 pSB3K3 not screened, its parent plasmid (A15-2) was wrong
K116002 (PnhaA+GFP) PnhaA-RBS-GFP-TT
 Sep 4 TOP10 pSB1A2 sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter
B5new2-3 (BBa_K173003) B1-B4 (RBS-pdc-RBS-adhB-TT) Sep 1 TOP10 pSB1AK3 length ok (digestion E-P), sequence ok -long part- (BMR)
B9-2 (BBa_K173021) F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT) Sep 4 TOP10 pSB1AK3 length ok (digestion E-P), sequence ok -long part- (BMR)
B10-2 (BBa_K173022) F2620-B6 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-RBS-pdc-TT) Sep 4 TOP10 pSB1AK3 length ok + 2 extra bands (digestion E-P), sequence ok -long part- (BMR)
B9new-1 to 5 F2620-B5new2(-3) (Ptet-RBS-luxR-TT-Plux-RBS-pdc-RBS-adhB-TT) Oct 1 TOP10 pSB1AK3 length ok (digestion E-P)
A19 F2620 Oct 1 TOP10 pSB4C5 length ok (digestion E-P)
A21 A19-B5new2(-3) Oct 8 TOP10 pSB4C5 length ok (digestion E-P)
A14S-3 (BBa_K173012) J23118-RBS-lacI-TT-Plac-RBS-GFP-TT (A11(-3)-E0240) ? TOP10 pSB1A2 length ok (digestion E-P)
A17new (BBa_K173025) Plac-RBS-GFP-TT (R0011-E0240) Sep 4 TOP10 pSB1A2 length ok (PCR), sequence ok (BMR)
A20 F2620-A8 (Ptet-RBS-luxR-TT-Plux-RBS-tetR-TT-Ptet-RBS-GFP-TT) Oct 8 TOP10 pSB1A2 length ok (digestion E-P)
I52002 (4C5) plasmid ? TOP10 pSB4C5 length ok (digestion E-P)
I714891 (3K3) plasmid Aug 21 TOP10 pSB3K3 length ok (digestion E-P)
B14 (BBa_K173023) F2620-B3 (Ptet-RBS-luxR-TT-Plux-RBS-pdc-TT) Oct 16 TOP10 pSB1A2 to screen
B15 (BBa_K173024) F2620-B4 (Ptet-RBS-luxR-TT-Plux-RBS-adhB-TT) Oct 16 TOP10 pSB1A2 to screen

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