Team:DTU Denmark/acknowledgement

From 2009.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 161: Line 161:
   <font color="#990000" face="arial" size="3">
   <font color="#990000" face="arial" size="3">
   <br>
   <br>
-
     Also check out our Danish website<br><br>
+
     Sponsors and acknowledgements<br><br>
-
  </font>
+
-
  <font color="DDDDDD" face="arial" size="2">
+
-
    <a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>- DTU iGEM 2009</a><br>
+
-
    <br>
+
-
  <font color="#990000" face="arial" size="3">
+
-
  <br>
+
-
    The people we owe it all to<br><br>
+
   <font color="DDDDDD" face="arial" size="2">
   <font color="DDDDDD" face="arial" size="2">
     <a href="https://2009.igem.org/Team:DTU_Denmark/sponsors" CLASS=leftbar>- List of sponsors</a><br>
     <a href="https://2009.igem.org/Team:DTU_Denmark/sponsors" CLASS=leftbar>- List of sponsors</a><br>
Line 177: Line 170:
   <font color="#990000" face="arial" size="5">
   <font color="#990000" face="arial" size="5">
<br>   
<br>   
-
<b>Acknowledgements</b><br><br><br>
+
<b>DTU denmark</b><br><br><br>
   </font>
   </font>
   <font color="#333333" face="arial" size="2.5">
   <font color="#333333" face="arial" size="2.5">
Line 184: Line 177:
<!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
<!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
-
<font size="4"><b>Thanks to all our advisors and people from whom we have received technical assistance</b></font><br><br>
+
<font size="4"><b>Acknowledgements</b></font><br><br>
 +
 
 +
<p align="justify">A very special thanks goes out to assistant professor <b>Kiran Raosaheb Patil</b> for getting the group together and for his expert guidance and advise.<br><br><p>
-
A very special thanks goes out to professor <b>Kiran Raosaheb Patil</b> for getting the group together and for his expert guidance and advise<br><br>
+
<p align="justify">Also a special thanks to PhD student <b>Simon Carlsen</b> for invaluable experimental advice and practical help, and to Post. Doc <b>Bjarne Gram Hansen</b> for sharing his expertise on USER fusion, and for his help constructing our redoxilator construct. We also owe thanks to Post. Doc. <b>Line Albertsen</b> for helping with the redoxilator construct, and for her input for design of protein fusions using linkers.<br><br><p>
-
Also a special thanks to <b>Simon Carlsen</b> for invaluable experimental advice and practical help, and to Post. Doc <b>Bjarne Gram Hansen</b> for sharing his expertise on USER fusion<br><br>
+
<p align="justify">Many thanks to associate professor <b>Chris Workman</b> and PhD student <b>Emmanouil Papadakis</b> for taking time out of their busy schedules to help us with GFP measurements.<br><br><p>
-
Thanks to <b>Marc Juul</b> for helping with the web interface and server uplink of the USER fusion primer design program.<br>
+
<p align="justify">Last but not least, thanks to master's student <b>Marc Juul Christoffersen</b> for helping with the web interface and server uplink of the USER fusion primer design program - Watch out for this guy at next years iGEM comp!<br><br><p>
-
- Watch out for this guy at next years iGEM comp!<br>
+
Line 198: Line 192:
  <td width="182px" height="100%" valign="top" class="greybox">
  <td width="182px" height="100%" valign="top" class="greybox">
   <font face="arial" size="2">
   <font face="arial" size="2">
-
  <p align="center"><b>Headline</b></p><br>
 
Line 204: Line 197:
 +
<p>
 +
<strong>Achievements</strong><br>
 +
<br>
 +
<strong>Redox sensing device</strong><br>
 +
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal. Extensive mathematical modelling was performed to simulate how the construct would operate <i>in vivo</i>.<br>
 +
<br>
 +
<strong>Biobricks</strong><br>
 +
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)<br>
 +
<br>
 +
We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)<br>
 +
<br>
 +
<strong>USER fusion Assembly standard</strong><br>
 +
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)<br>
 +
<br>
 +
<strong>USER-fusion primer design software</strong><br>
 +
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.
 +
</p>

Latest revision as of 10:36, 21 October 2009

Wiki banner 967px.png

Welcome to the DTU iGEM wiki!


Sponsors and acknowledgements

- List of sponsors
- Acknowledgement

DTU denmark


Acknowledgements

A very special thanks goes out to assistant professor Kiran Raosaheb Patil for getting the group together and for his expert guidance and advise.

Also a special thanks to PhD student Simon Carlsen for invaluable experimental advice and practical help, and to Post. Doc Bjarne Gram Hansen for sharing his expertise on USER fusion, and for his help constructing our redoxilator construct. We also owe thanks to Post. Doc. Line Albertsen for helping with the redoxilator construct, and for her input for design of protein fusions using linkers.

Many thanks to associate professor Chris Workman and PhD student Emmanouil Papadakis for taking time out of their busy schedules to help us with GFP measurements.

Last but not least, thanks to master's student Marc Juul Christoffersen for helping with the web interface and server uplink of the USER fusion primer design program - Watch out for this guy at next years iGEM comp!

Achievements

Redox sensing device
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal. Extensive mathematical modelling was performed to simulate how the construct would operate in vivo.

Biobricks
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)

We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)

USER fusion Assembly standard
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)

USER-fusion primer design software
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.

Comments or questions to the team? Please -- Comments of questions to webmaster? Please