Team:HKUST/Result3

From 2009.igem.org

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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensoring">Odorant Sensoring</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttranctantProduction">Attranctant Production</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>
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<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li>
<li><a href="https://2009.igem.org/Team:Gallery">Gallery</a></li>
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<li><a href="https://2009.igem.org/Team:Consolidation">Consolidation</a></li>
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<li><a href="https://2009.igem.org/Team:Biosafety">Biosafety</a></li>
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li>
<li><a href="https://2009.igem.org/Team:Acknowledgement">Acknowledgement</a></li>
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<p>Constructs </p>
<p>Constructs </p>
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   We have successfully cloned the FUS1t gene into the multiple cloning site of the plasmid pRS426. The agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1 is shown in Figure 6. The other parts, the FUS1 promoter and the EGFP gene are still under construction.  Also, the ARO9 gene has been amplified from yeast genome.</p>
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   We have successfully cloned the FUS1t gene into the multiple cloning site of the vector pRS426. Agarose gel electrophoresis shows correct sizes after enzyme digestion of pRS426-tFUS1. The other parts, the FUS1 promoter and the EGFP gene are still under construction.  Also, the ARO9 gene has been amplified from the yeast genome.</p>
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Figure 6 agarose gel electrophoresis pictures showing the correct sizes after enzyme digestion of pRS426-tFUS1
 
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Latest revision as of 19:06, 21 October 2009

Salt and Soap template

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Constructs

We have successfully cloned the FUS1t gene into the multiple cloning site of the vector pRS426. Agarose gel electrophoresis shows correct sizes after enzyme digestion of pRS426-tFUS1. The other parts, the FUS1 promoter and the EGFP gene are still under construction. Also, the ARO9 gene has been amplified from the yeast genome.



  • Background
  • Experimental Design
  • Parts Design
  • Experimental Results
  • Future Work
  • References
  • HKUST