Team:IIT Madras/Notebook/Sandbox

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Latest revision as of 01:30, 22 October 2009

Indian Institute of Technology,Madras

Intermediates and proposed constructs for the the 2 plasmid locking system

Original design

In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).

Plasmid 1

Plasmid 2

Final design

We decided to remove the extra antibiotic markers from the inserts. Then it looks like this

Plasmid 1

We had more than one way to build plasmid 1

Path 1

Plasmid 2

Intermediates and proposed constructs for the the 3 plasmid locking system

We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.

Intermediates planned through PCR protocol

These parts are essentially same as the those that occur in the construction strategies that are shown above.

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+{{:Team:IITM/main}}
-== Intermediates and final constructs planned for the the 2 plasmid locking system ==
+<html>
+<br><br><br><br><br><br><br><br><br><br><br>
+<br><br>
+<head><style>
+body
+{
+ background: #000;
+}
-== Intermediates and final constructs planned for the the 3 plasmid locking system ==
+#aContent
+{
+ background-color:#fff;
+ margin: 50px auto;
+ padding: 10px 10px 60px 10px;
+ width: 800px;
+ color: #000;
+}
+</style>
-== intermediates planned through PCR protocol ==
+<STYLE TYPE="text/css">
+<!--
+.justifyalign {text-align:justify}
+-->
+</STYLE>
+</head>
-== Plasmids for a self locking assembly ==
+<body>
+<div id="aContent">
+</html>
+==Intermediates and proposed constructs for the the 2 plasmid locking system==
+==Original design==
+In this design, each of the construct has its own antibiotic resistance marker (independent of the plasmid backbone marker).
+<html><br></html>
+<b><font color="#000">Plasmid 1</font></b>
+<html><br></html>
+[[Image:2plasmidoriginaldesignplasmid1.jpg]]
+<html><br></html>
+<b><font color="#000">Plasmid 2</font></b>
+<html><br></html>
+[[Image:2plasmidoriginaldesignplasmid2.jpg]]
+<html><br></html>
+==Final design==
+We decided to remove the extra antibiotic markers from the inserts. Then it looks like this
+<html><br></html>
+<b><font color="#000">Plasmid 1</font></b>
+<html><br></html>
+We had more than one way to build plasmid 1
+<html><br></html>
+<b><font color="#000">Path 1</font></b>
+[[Image:2plasmidfinaldesignplasmid1path1.jpg]]
+<html><br></html>
+<b><font color="#000">Path 1</font></b>
+[[Image:2plasmidfinaldesignplasmid1path2.jpg]]
+<html><br></html>
+<b><font color="#000">Plasmid 2</font></b>
+<html><br></html>
+[[Image:2plasmidfinaldesignplasmid2.jpg]]
+<html><br></html>
+==Intermediates and proposed constructs for the the 3 plasmid locking system==
+We have designed the constructs for the 3 plasmid system, but we haven't planned to build it yet. Some of the parts built for the 2 plasmid system could be reused for the 3 plasmid system too.
+== Intermediates planned through PCR protocol ==
+<html><br></html>
+These parts are essentially same as the those that occur in the construction strategies that are shown above.
+{{:Team:IITM/footer}}