From 2009.igem.org
(Difference between revisions)
|
|
| (37 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:BCCS-Bristol/Header}}
| |
| | | | |
| - | =====Abstract=====
| |
| - | We wanted to exploit the fact that outer membrane vesicles (OMVs) were naturally produced in gram negative bacteria such as E. coli. The aim was to use them in our advantage as a directed delivery system to cells of proteins of our interest.This can potentially be used for the ''safe'' delivery of drugs into cells or for cell-cell communication purposes.We believe that the inclusion of proteins in vesicles will not only protect them from degradation in the extracellular environment but will also protect other cells if the cargo proteins packaged into vesicles are toxic or harmful.
| |
| - |
| |
| - | Because the exact mechanism by which OMVs are produced in gram negative cells is not yet elucidated (although 3 models have been proposed, Lauren M. Mashburn-Warren ''et al'', 2006) we decided to make protein fusions of proteins that were normally included in OMVs with proteins of our interest. Experiments with protein fusions to the toxin ClyA were already made with success(Jae-Young Kim ''et al'', 2008) and the desired proteins were delivered to OMVs. But for the purposes that we would be using OMVs we thought that it would be safer to use harmless proteins that would act as carriers instead of ClyA. Hence we selected 3 possible candidate protein carriers (Eun-Young Lee ''et al'', 2007) to be used in our project.These proteins are:
| |
| - | 1. OsmE (''Osm''otically inducible lipoprotein ''E'')
| |
| - | 2. fiu (siderophore receptor)
| |
| - | 3. FhuA (Ferrichrome-iron receptor)
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | [https://2009.igem.org/Team:BCCS-Bristol/Notebook/Lab_Book '''Lab Book''']
| |
| - |
| |
| - |
| |
| - | [https://2009.igem.org/Team:BCCS-Bristol/Notebook/Biobricks Biobricks]
| |
Latest revision as of 00:01, 21 October 2009
"
