Wisconsin-Madison/28 September 2009

From 2009.igem.org

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===='''July , 2009'''====
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===='''September 28, 2009'''====
set up assay to compare and contrast growth in Salt Free LB and Minimal Media, also observe effects of placing GsSDMT on pBAD18
set up assay to compare and contrast growth in Salt Free LB and Minimal Media, also observe effects of placing GsSDMT on pBAD18
Line 67: Line 67:
double transformation of 33/GSMT and 18/GsSDMT
double transformation of 33/GSMT and 18/GsSDMT
 +
developed assay for testing both genes
developed assay for testing both genes
 +
 +
 +
Digestion of cloning, pBAD35, photopromoter, MetK+Pbad35

Latest revision as of 19:57, 20 October 2009

Calendar

April
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_April_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_April_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_April_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_April_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_April_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_April_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_April_2009 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_April_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_April_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_April_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_April_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_April_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/13_April_2009&action=edit 13] [http://2009.igem.org/Wisconsin-Madison/14_April_2009 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_April_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_April_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/17_April_2009&action=edit 17] [http://2009.igem.org/Wisconsin-Madison/18_April_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_April_2009 19]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/20_April_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_April_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_April_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_April_2009&action=edit 23] [http://2009.igem.org/Wisconsin-Madison/24_April_2009 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_April_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_April_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_April_2009&action=edit 27] [http://2009.igem.org/Wisconsin-Madison/28_April_2009 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_April_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_April_2009&action=edit 30]
May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/5_May_2009&action=edit 5] [http://2009.igem.org/Wisconsin-Madison/6_May_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/12_May_2009&action=edit 12] [http://2009.igem.org/Wisconsin-Madison/13_May_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_May_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/15_May_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/16_May_2009&action=edit 16] [http://2009.igem.org/Wisconsin-Madison/17_May_2009 17]
[http://2009.igem.org/Wisconsin-Madison/18_May_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_May_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_May_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_May_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_May_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_May_2009&action=edit 24]
[http://2009.igem.org/Wisconsin-Madison/25_May_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_May_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_May_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_May_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_May_2009 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_May_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Wisconsin-Madison/1_June_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_June_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_June_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_June_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_June_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_June_2009 6] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/7_June_2009&action=edit 7]
[http://2009.igem.org/Wisconsin-Madison/8_June_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_June_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_June_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_June_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_June_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/14_June_2009&action=edit 14]
[http://2009.igem.org/Wisconsin-Madison/15_June_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_June_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_June_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_June_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_June_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_June_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_June_2009 21]
[http://2009.igem.org/Wisconsin-Madison/22_June_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_June_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_June_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_June_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_June_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_June_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_June_2009 28]
[http://2009.igem.org/Wisconsin-Madison/29_June_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Wisconsin-Madison/1_July_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_July_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_July_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_July_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_July_2009 5]
[http://2009.igem.org/Wisconsin-Madison/6_July_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_July_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_July_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_July_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_July_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_July_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_July_2009 12]
[http://2009.igem.org/Wisconsin-Madison/13_July_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_July_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_July_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_July_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/19_July_2009&action=edit 19]
[http://2009.igem.org/Wisconsin-Madison/20_July_2009 20] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_July_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_July_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_July_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/Wisconsin-Madison/1_August_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_August_2009 2]
[http://2009.igem.org/Wisconsin-Madison/3_August_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_August_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_August_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_August_2009 6] [http://2009.igem.org/Wisconsin-Madison/7_August_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_August_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_August_2009 9]
[http://2009.igem.org/Wisconsin-Madison/10_August_2009 10] [http://2009.igem.org/Wisconsin-Madison/11_August_2009 11] [http://2009.igem.org/Wisconsin-Madison/12_August_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_August_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_August_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_August_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_August_2009 16]
[http://2009.igem.org/Wisconsin-Madison/17_August_2009 17] [http://2009.igem.org/Wisconsin-Madison/18_August_2009 18] [http://2009.igem.org/Wisconsin-Madison/19_August_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_August_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_August_2009 21] [http://2009.igem.org/Wisconsin-Madison/22_August_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_August_2009 23]
[http://2009.igem.org/Wisconsin-Madison/24_August_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_August_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_August_2009 26] [http://2009.igem.org/Wisconsin-Madison/27_August_2009 27] [http://2009.igem.org/Wisconsin-Madison/28_August_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_August_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_August_2009 30]
[http://2009.igem.org/Wisconsin-Madison/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Wisconsin-Madison/1_September_2009 1] [http://2009.igem.org/Wisconsin-Madison/2_September_2009 2] [http://2009.igem.org/Wisconsin-Madison/3_September_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_September_2009 4] [http://2009.igem.org/Wisconsin-Madison/5_September_2009 5] [http://2009.igem.org/Wisconsin-Madison/6_September_2009 6]
[http://2009.igem.org/Wisconsin-Madison/7_September_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_September_2009 8] [http://2009.igem.org/Wisconsin-Madison/9_September_2009 9] [http://2009.igem.org/Wisconsin-Madison/10_September_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_September_2009&action=edit 11] [http://2009.igem.org/Wisconsin-Madison/12_September_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_September_2009 13]
[http://2009.igem.org/Wisconsin-Madison/14_September_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_September_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_September_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_September_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_September_2009&action=edit 18] [http://2009.igem.org/Wisconsin-Madison/19_September_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_September_2009 20]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/21_September_2009&action=edit 21] [http://2009.igem.org/Wisconsin-Madison/22_September_2009 22] [http://2009.igem.org/Wisconsin-Madison/23_September_2009 23] [http://2009.igem.org/Wisconsin-Madison/24_September_2009 24] [http://2009.igem.org/Wisconsin-Madison/25_September_2009 25] [http://2009.igem.org/Wisconsin-Madison/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_September_2009&action=edit 27]
[http://2009.igem.org/Wisconsin-Madison/28_September_2009 28] [http://2009.igem.org/Wisconsin-Madison/29_September_2009 29] [http://2009.igem.org/Wisconsin-Madison/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Wisconsin-Madison/1_October_2009 1] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/2_October_2009&action=edit 2] [http://2009.igem.org/Wisconsin-Madison/3_October_2009 3] [http://2009.igem.org/Wisconsin-Madison/4_October_2009 4]
[http://2009.igem.org/Wisconsin-Madison/5_October_2009 5] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/6_October_2009&action=edit 6] [http://2009.igem.org/Wisconsin-Madison/7_October_2009 7] [http://2009.igem.org/Wisconsin-Madison/8_October_2009 8] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/9_October_2009&action=edit 9] [http://2009.igem.org/Wisconsin-Madison/10_October_2009 10] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/11_October_2009&action=edit 11]
[http://2009.igem.org/Wisconsin-Madison/12_October_2009 12] [http://2009.igem.org/Wisconsin-Madison/13_October_2009 13] [http://2009.igem.org/Wisconsin-Madison/14_October_2009 14] [http://2009.igem.org/Wisconsin-Madison/15_October_2009 15] [http://2009.igem.org/Wisconsin-Madison/16_October_2009 16] [http://2009.igem.org/Wisconsin-Madison/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/18_October_2009&action=edit 18]
[http://2009.igem.org/Wisconsin-Madison/19_October_2009 19] [http://2009.igem.org/Wisconsin-Madison/20_October_2009 20] [http://2009.igem.org/Wisconsin-Madison/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Wisconsin-Madison/29_October_2009&action=edit 29] [http://2009.igem.org/Wisconsin-Madison/30_October_2009 30] [http://2009.igem.org/Wisconsin-Madison/31_October_2009 31]

September 28, 2009

set up assay to compare and contrast growth in Salt Free LB and Minimal Media, also observe effects of placing GsSDMT on pBAD18

200uL total:

.2 amp

20uL cells grown to .4 OD 600

either:

180uL media

164uL media 16uL 5M NaCl

or

156uL media 24uL 5M NaCl

.2uL DMG

.15uL 15% arabinose


double transformation of 33/GSMT and 18/GsSDMT

developed assay for testing both genes


Digestion of cloning, pBAD35, photopromoter, MetK+Pbad35

Retrieved from "http://2009.igem.org/Wisconsin-Madison/28_September_2009"