Team:HKUST/Protocols
From 2009.igem.org
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- | <li><a href="https://2009.igem.org/Team:HKUST/ | + | <li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li> |
- | <li><a href="https://2009.igem.org/Team:HKUST/ | + | <li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li> |
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li> | <li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li> | ||
Latest revision as of 09:53, 20 October 2009
a
- Agarose gel preparation and gel electrophoresis
- PCR (Taq, Vent)
- PCR product clean-up
- Enzyme digestion (vector, insert)
- Plasmid DNA extraction
- Gel cutting
- Gel extraction
- Ethanol precipitation
- Ligation
- Transformation to E.coli
- Transformation to yeast
- Cell lysis
- Yeast genomic DNA extraction
- Western blotting
- GFP testing